Cysteine proteinases, production and use

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases

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4241951, 424DIG13, 435219, A61K 3754

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active

051066211

ABSTRACT:
A cysteine proteinase characterized in that it is immunologically distinct, has a molecular mass of about 25,000 and has a distinct net charge is disclosed. "Ananain" and "comosain" are such enzymes, which may be distinguished by their specificities for certain synthetic substrates.
A process for the production thereof is also disclosed which comprises separation from pineapple plant material and purification. Such enzymes may be used in the debridement of eschar tissues.

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patent: 4645668 (1987-02-01), Pinnell
Klein, "Enzymatic Debridement of Third Degree Burns in Aminals with Bromelains--A Preliminary Report", J. of the Maine Medical Association, Sep. 1964.
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Rowan et al., "Ananain: A Novel Cysteine Proteinase Found in Pineapple Stem," Archives of Biochem. and Biophys., 267: 262-270 (1988).
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Biochem. J., vol. 221, 1984, pp. 445-452, GB; A. D. Gounaris et al., Human Plasma Alpha-Cysteine Proteinase Inhibitor.

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