Cyclosporine assay

Details Cyclosporine assay Cyclosporine assay

1993-01-12

1996-01-02

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 18, 435212, 435213, 435233, 436512, 530317, 530330, 530331, C12Q 137, C07K 500

Patent

active

054807794

ABSTRACT:
A process for determining the concentration of cyclosporine in a sample, which comprises adding to the sample a predetermined amount of an isomerase which isomerizes N-terminal peptide to proline, then measuring the enzyme-catalyzed inhibition of the isomerization of a proline-containing substrate, and determining the concentration or cyclosporine from a calibration curve.

REFERENCES:
patent: 4592997 (1986-06-01), Wilhelms et al.
G. Fischer, Nature, vol. 337, Feb. 2, 1989, pp. 476-478.
G. Fischer, Biomed. Biochim. Acta 43 (1984) 10, 1101-1111.
E. R. Schonbrunner, J. Of Bio. Chem., vol. 266 (1991), pp. 1-2.
P. M. Kabra, Clin. Chem. 31/12, (1987), pp. 2272-2274.
R. K. Harrison, Biochemistry, vol. 29, No. 16 (1990), pp. 3813-3816.
H. P. Bachinger, J. of Bio. Chem., vol. 262, No. 35 (1987), pp. 17144-17149.
P. A. Keown, Trans. Pro., vol. XV, No. 4, Suppl. 1 (1983) pp. 2438-2441.
Woloszczuk, Chemical Abstracts, vol. 110, pp. 84, Ref. No. 166166y, 1989.
Takahashi et al, Chemical Abstracts, vol. 113, p. 318, Ref. No. 227149f, 1990.
Kofron et al, Biochemistry, vol. 30, No. 25, pp. 6127-6134, 1991.
Sigal et al, J. Exp. Med., vol. 173, pp. 619-628, Mar. 1991.

Affiliated with

Also associated with

Rating

Cyclosporine assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cyclosporine assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cyclosporine assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-234395