Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1997-10-09
1999-12-21
Sisson, Bradley
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4353201, 536 232, C12N 910, C12N 1500, C07H 2104
Patent
active
060047909
ABSTRACT:
The present invention relates to variants of cyclomaltodextrin glucanotransferase. More specifically the invention relates to a method of modifying the substrate binding and/or product selectivity of a precursor CGTase enzyme, and CGTase variants derived from a precursor CGTase enzyme by substitution, insertion and/or deletion of one or more amino acid residue(s), which amino acid residue(s) holds a position close to the substrate. Moreover, the invention relates to DNA constructs encoding the CGTase variants, expression vectors, host cells and methods of producing the CGTase variants of the invention.
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Andersen Carsten
Dijkhuizen Lubbert
Dijkstra Bauke W.
Osten Claus von der
Lambiris, Esq. Elias J.
Novo Nordisk A S
Sisson Bradley
Stole Einar
Zelson Esq. Steve T.
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