Cycloheximide resistant gene

Details Cycloheximide resistant gene Cycloheximide resistant gene

1985-12-12

1989-08-15

Teskin, Robin

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4351721, 4351723, 435320, 435255, 536 27, C12N 500, C12N 1500, C12P 1934

Patent

active

048574600

ABSTRACT:
A cycloheximide resistant gene of the present invention can be produced by cultivating yeast belonging to Candida genus and by cleaving a cycloheximide resistant gene from the yeast using restriction enzymes XbaI and Sau3AI.
By incorporating the cycloheximide resistant gene together with the gene for useful substance into a plasmid, the transformant producing useful substance can be readily detected.

REFERENCES:
Takagi et al., Jun. 1985, J. Gen. Appl. Micro., vol. 31 (3), pp. 267-276, "Induction of cycloheximide resistive on Candida maltosa by modifying the ribosomes".
Kaufer et al., Nucl. Acids Res., vol. 10 (10), 1983, pp. 3123-3135, "Cycloheximide resistance in yeast: the gene and its protein".
Fried et al., Nucl. Acids Res., vol. 10 (10), 1982, pp. 3133-3148, "Molecular cloning and analysis of yeast gene for cycloheximide resistance and ribosomal protein L29".
Gillum et al., Mol Gen. Genet., vol. 198, pp. 179-182, 1984, "Isolation of the Candida albicans gene for orotidine-5-phosphate decarboxylase by complementation".
Brown et al., Methods Enzymol, vol. 101, pp. 278-290, 1983, "Yeast vectors with negative selection".
Takagi et al., J. Bact., Oct. 1986, pp. 417-419, vol. 168 (1), "Cloning in Saccharomyces cereviviace of a cycloheximide resistance gene from the Candida maltosa gene which modifies ribosomal".

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