Cycling DNA/RNA amplification electrochemiluminescent probe assa

Details Cycling DNA/RNA amplification electrochemiluminescent probe assa Cycling DNA/RNA amplification electrochemiluminescent probe assa

1995-06-07

2000-04-11

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 911, 435 912, 536 243, 536 2433, C07H 2104, C12Q 168, C12P 1934

Patent

active

060486872

ABSTRACT:
This invention relates to an improved process for detecting and quantifying a desired nucleic acid sequence. The process involves synthesizing single stranded RNA, single stranded DNA, double-stranded DNA followed by detection using an electrochemiluminescent labeled binding species.

REFERENCES:
patent: 5679519 (1997-10-01), Oprandy
Gemen et al., "A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescence (ECL) labelled probes", Journal of Virological Methods, vol. 49, pp. 157-168, Feb. 14, 1994.
Guatelli, et al. Isothermal, in vitro Amplification of nucleic acids by a multienzyme reaction . . . Proc.Natl.Acad.Sci USA (Mar. 1990) 87:1874-1878.
Fahy et al. Self-Sustained Sequence Replication (35R): An Isothermal Transcription--Based Amplification . . . PCR Methods and Applications (Aug. 1991) 1:25-33.
Becker-Andre et al. Absolute mRNA quantification using the polymerase chain reaction (PCR), A Novel . . . Nucl.Acids Res. (1989) 17:9437-9446.
Blackburn et al. Electrochemiluminescence Detection for Development of Immunoassays . . . Clin Chem (1991) 37:1534-1539.

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