Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1996-09-05
1998-06-09
Degen, Nancy
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 4353201, 435325, 530350, 536 235, 536 2431, C07H 2100, C12N 1500, C12N 1512, C12N 1563
Patent
active
057632197
ABSTRACT:
The present invention is directed to nucleic acid molecules encoding a truncated human cyclin E protein, the truncated human cyclin E protein being a constitutively active form of human cyclin E protein. These truncated forms can be encoded by the nucleotide sequence of wild-type cyclin E, with a deletion therein to result in the truncated protein. Vectors and host cells containing the nucleic acid molecules are also provided. The invention further provides isolated fragments of the truncated cyclin E proteins, which fragments consist essentially of the deletion flanking regions of the wild-type cyclin E nucleotide sequence. Antisense nucleic acid molecules, and fragments thereof, to the truncated cyclin E protein and to the fragments thereof are also provided. Methods using the nucleic acid molecules, fragments thereof, antisense nucleic acid molecules, and fragments thereof, are provided. The antisense can be used therapeutically for inhibition of cyclin E activity. An isolated truncated human cyclin E protein is also provided which is a constitutively active form of human cyclin E protein, as well as fragments of the isolated truncated protein. Antibodies to the protein and fragment of the protein are provided, as are compositions comprising the protein and fragment of the protein. Methods using the proteins and fragments of the protein are provided.
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Degen Nancy
Health Research Incorporated
Larson Thomas G.
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