Cyclin dependent kinase mutants, and diagnostic and...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S007720

Reexamination Certificate

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06566054

ABSTRACT:

BACKGROUND OF THE INVENTION
The cell division cycle is one of the most fundamental processes in biology which, in multicellular organisms, ensures the controlled generation of cells with specialized functions. Under normal growth conditions, cell proliferation is tightly regulated in response to diverse intra-and extracellular signals. This is achieved by a complex network of protooncogenes and tumor-suppresser genes that are components of various signal transduction pathways. Activation of a protooncogene(s) and/or a loss of a tumor suppresser gene(s) can lead to the unregulated activity of the cell cycle machinery. This, in turn, will lead to unregulated cell proliferation and to the accumulation of genetic errors which ultimately will result in the development of cancer (Pardee,
Science
246:603-608, 1989).
In the eukaryotic cell cycle a key role is played by the cyclin-dependent kinases (CDKs). CDK complexes are formed via the association of a regulatory cyclin subunit and a catalytic kinase subunit. In mammalian cells, the combination of the kinase subunits (such as cdc2, CDK2, CDK4 or CDK6) with a variety of cyclin subunits (such as cyclin A, B1, B2, D1, D2, D3 or E) results in the assembly of functionally distinct kinase complexes. The coordinated activation of these complexes drives the cells through the cell cycle and ensures the fidelity of the process (Draetta,
Trends Biochem. Sci.
15:378-382, 1990; Sherr,
Cell
73:1059-1065, 1993). Each step in the cell cycle is regulated by a distinct and specific cyclin-dependent kinase. For example, complexes of CDK4 and D-type cyclins govern the early G1 phase of the cell cycle, while the activity of the CDK2/cyclin E complex is rate limiting for the G1 to S-phase transition. The CDK2/cyclin A kinase is required for the progression through S-phase and the cdc2/cyclin B complex controls the entry into M-phase (Sherr,
Cell
73:1059-1065, 1993).
The CDK complex activity is regulated by mechanisms such as stimulatory or inhibitory phosphorylations as well as the synthesis and degradation of the kinase and cyclin subunits themselves. Recently, a link has been established between the regulation of the activity of cyclin-dependent kinases and cancer by the discovery of a group of CDK inhibitors including the p16
Ink4a
, p15
Ink4b
, p18
Ink4c
, p19/p20
Ink4d
, p21
Waf1/Cip1
, p27
Kip1
and p57
kip2
proteins. The activity of p21 is regulated transcriptionally by DNA damage through the induction of p53, senescence and quiescence (Harper et al.,
Cell
75:805-816, 1993). The inhibitory activity of p27 is induced by the negative growth factor TGF-&bgr; and by contact inhibition (Polyak et al.,
Cell
78:66-69, 1994). These proteins, when bound to CDK complexes, inhibit their kinase activity, thereby inhibiting progression through the cell cycle. Although their precise mechanism of action is unknown, it is thought that binding of these inhibitors to the CDK/cyclin complex prevents its activation. Alternatively, these inhibitors may interfere with the interaction of the enzyme with its substrates or its cofactors.
While p21 and p27 inhibit all the CDK/cyclin complexes tested, the Ink4 proteins, e.g., p16, p15, p18 and p19 block exclusively the activity of the CDK4/cyclin D and CDK6/cyclin D complexes in the early G1 phase (Serrano et al.,
Nature
366:704-707, 1993), by either preventing the interaction of CDK4 and Cyclin D1, or indirectly preventing catalysis. As mentioned above, the p21 is positively regulated by the tumor suppresser p53 which is mutated in approx. 50% of all human cancers. p21 may mediate the tumor suppresser activity of p53 at the level of cyclin-dependent kinase activity. p16 is the product of a tumor suppresser gene localized to the 9p21 locus, which is frequently mutated in human cancer cells.
Of all the various kinases, the CDK4/cyclin D complexes are known to play an important role in regulating cell cycle progression in early G1. These complexes function as integrators of various growth factor-induced extracellular signals and as a link between the different signal transduction pathways and other cyclin-dependent kinases. The expression of the cyclin D1 positive regulatory subunit, is deregulated by gene translocations, retroviral insertions and amplifications in parathyroid adenomas, lymphomas, esophageal and breast carcinomas. The targeted overexpression of cyclin D1 in the mammary epithelium of transgenic mice induces mammary adenomas and adenocarcinomas. This confirms that cyclin D1, when overexpressed, acts as an oncogene (Wang et al.,
Nature
369:669-671, 1994). Moreover p16 is deleted at high frequency in cell lines derived from tumors of lung, breast, brain, bone, skin, bladder, kidney, ovary, and lymphocyte. These data support the idea that the lack of functional p16 or the overexpression of cyclin D1 leads to the deregulation of CDK4/cyclin D1 kinase activity and thereby contribute to uncontrolled cell proliferation.
SUMMARY OF THE INVENTION
One aspect of the present invention relates to diagnostic assays for determining, in the context of cells isolated from a patient, if mutations have arisen in one or more cyclin dependent kinases of the sample cells. In particular, the assay assesses CDK genes and gene products for mutations that render the a cyclin dependent kinase insensitive to the inhibitory activity of a CKI protein, e.g., an Ink4 protein or a CIP protein. In general, the assay of the instant application detects mutations which alter (e.g., decrease) the association constant (Ka) between a CDK/CKI protein complex. As described herein, the subject assay provides a method for determining if an animal is at risk for a disorder characterized by aberrant cell proliferation, differentiation and/or apoptosis.
Yet another aspect of the present invention relates to the modification of tumor cells, and/or the immune response to tumor cells in a patient by administering a vaccine to enhance the anti-tumor immune response in a host. The present invention provides, for examples, tumor vaccines based on administration of expression vectors encoding a mutant CDK protein, or portion thereof, or immunogenic preparations of polypeptides derived from mutant CDK. In still other embodiments, the tumor vaccine strategy can use the tumor cell itself as a source of tumor antigen.
The practice of the present invention will employ, unless otherwise indicated, conventional techniques of cell biology, cell culture, molecular biology, transgenic biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See, for example,
Molecular Cloning A Laboratory Manual,
2nd Ed., ed. by Sambrook, Fritsch and Maniatis (Cold Spring Harbor Laboratory Press:1989);
DNA Cloning,
Volumes I and II (D. N. Glover ed., 1985);
Oligonucleotide Synthesis
(M. J. Gait ed., 1984); Mullis et al. U.S. Pat. No. 4,683,195;
Nucleic Acid Hybridization
(B. D. Hames & S. J. Higgins eds. 1984);
Transcription And Translation
(B. D. Hames & S. J. Higgins eds. 1984);
Culture Of Animal Cells
(R. I. Freshney, Alan R. Liss, Inc., 1987);
Immobilized Cells And Enzymes
(IRL Press, 1986); B. Perbal,
A Practical Guide To Molecular Cloning
(1984); the treatise,
Methods In Enzymology
(Academic Press, Inc., N.Y.);
Gene Transfer Vectors For Mammalian Cells
(J. H. Miller and M. P. Calos eds., 1987, Cold Spring Harbor Laboratory);
Methods In Enzymology,
Vols. 154 and 155 (Wu et al. eds.),
Immunochemical Methods In Cell And Molecular Biology
(Mayer and Walker, eds., Academic Press, London, 1987);
Handbook Of Experimental Immunology,
Volumes I-IV (D. M. Weir and C. C. Blackwell, eds., 1986);
Manipulating the Mouse Embryo,
(Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986).


REFERENCES:
Serrano et al “A New Regulatory Motif in Cell Cycle Control Causing Specific Inhibition of Cyclin D CDK4”. Nature vol. 366: 704-707, Dec. 16, 1993.*
Xiong et al “p21 is a Universal Inhibitor of Cyclin Kinases” Nature vol. 366: 701-704, Dec. 16, 1993.*
Mor

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