Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1993-07-12
1995-07-25
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 6, 435 29, 435 39, 435 4, 436 94, 436800, 536 2673, 536 111, 536 256, C12Q 104, C12Q 168, G01N 3300, C07H 100
Patent
active
054361346
ABSTRACT:
The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. In particular, the invention describes unsymmetrical cyanine dyes having a saturated or unsaturated cyclic substituent. The dyes of the invention possess superior fluorescent characteristics when complexed with nucleic acids, and have utility in any application which requires detection of nucleic acids. The presence of the cyclic substituent results in improved permeability in a wide range of living cells, resulting in improved detection of intracellular nucleic acids.
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Haugland Richard P.
Millard Paul J.
Roth Bruce L.
Yue Stephen T.
Helfenstein Allegra J.
Leary Louise N.
Molecular Probes, Inc.
Skaugset Anton E.
Wityshyn Michael G.
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