Cyclic nucleotide-gated cation channel subunit 3B (CNG3B)...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C514S002600, C514S012200, C536S023500, C435S069100

Reexamination Certificate

active

07371823

ABSTRACT:
The invention provides isolated nucleic acid and amino acid sequences of CNG3B, antibodies to CNG3B, methods of detecting CNG3B, and methods of screening for modulators of cyclic nucleotide-gated ion channels using biologically active CNG3B. The invention further provides, in a computer system, a method of screening for mutations of human CNG3B genes as well as a method for identifying a three-dimensional structure of human CNG3B polypeptides.

REFERENCES:
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Gerstner, Andrea et al.; “Molecular Cloning and Functional Characterization of a New Modulatory Cyclic Nucleotide-Gated Channel Subunit from Mouse Retina”; 2000,The Journal of Neuroscience, vol. 20, No. 4, pp. 1324-1332.
Database EMBL, Jul. 6, 2000, retrived from EMBL Database accession No. AF228520.
Kohl, et al., “Mutations in the CNGB3 gene encoding the β-subunit of the cone photoreceptor cGMP-gated channel are responsible for achromatopsia (ACHM3) linked to chromosome 8q21”;Human Molecular Genetics, vol. 9, No. 14 2107-2116 (2000).
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Kramer et al. (2001). Modulation of cyclic-nucleotide-gated channels and regulation of vertebrate phototransduction. The Journal of Experimental Biology. 204:2921-2931.
Peng et al. (2003). Achromatopsia-associated mutation in the human cone photoreceptor cyclic nucleotide-grated channel CNG3B subunit alters the ligand sensitivity and pore properties of heteromeric channels. J. Biol. Chem. 278(36):34533-34540.
Broillet et al. (1999). Cyclin nucleotide-gated channels. Molecular mechanisms of activation. Annals New York Academy of Sciences. 868:730-40.
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