Cyclic maltosylmaltose, cyclic maltosymaltose-forming...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

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C435S007400, C435S015000, C435S041000, C536S123100, C536S124000

Reexamination Certificate

active

07951530

ABSTRACT:
An object of the present invention is to provide an option of non-reducing saccharide by providing a novel non-reducing saccharide composed of glucose as constituents and to provide a novel enzyme forming the non-reducing saccharide, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the non-reducing saccharide, and uses thereof. The present invention solves the above object by providing a novel cyclic saccharide having a structure of cyclo{>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>6)-α-D-glucopyranosyl-(1>4)-α-D-glucopyranosyl-(1>}, cyclic maltosylmaltose, novel cyclic maltosylmaltose-forming enzyme, a method and process for producing the same, a DNA encoding the enzyme, a recombinant DNA and transformant comprising the DNA, a composition comprising the cyclic maltosylmaltose or a saccharide composition comprising the same, and uses thereof.

REFERENCES:
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patent: WO 02/055708 (2002-07-01), None
Dexter French et a., “Studies on the Schardinger Dextrins. The Preparation and Solubility Characteristics of Alpha, Beta and Gamma Dextrins1”, vol. 71(1), pp. 353-356, Jan. 1949.
Kazuhisa Mukai; An enzymatically produced novel cyclic tetrasacchride, Cyclo-{→6)-α-D-Gicp-(1 →4)-α-D-Glcp-(1→6)-α-d-Glcp-(1→4)-α-D-Glcp(1→} (cyclic maltosyl-(1→6)-maltose), from starch; Carbohydrate Rearch, 340 (2005); pp. 1469-1474.
Kazuhisa Mukai; Purification, Characterization, and Gene Cloning of a Novel Maltosyltransferase from anArthrobacter globfformisStrain That Produces and Alternating α-1,4- and α-1,6-Cyclic Tetrasaccharide from Starch; Applied and Environmental Microbiology; vol. 72, No. 2; pp. 1065-1071; Feb. 2006.
Aga et al., Production of cyclictetrasaccharide from starch using a novel enzyme system fromBacillus globisporusC11. J. Biosci. Bioeng., 2002, vol. 94(4): 336-342.

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