Cycle sequencing with non-thermostable DNA polymerases

Details Cycle sequencing with non-thermostable DNA polymerases Cycle sequencing with non-thermostable DNA polymerases

1993-03-30

1995-07-11

Parr, Margaret

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 435 9121, 435 9132, 435 6, 536 231, 536 2433, 935 8, 935 16, 935 77, C12P 1934, C12Q 168, C07H 1700

Patent

active

054320658

ABSTRACT:
Method for performing a cycled primer extension reaction by contacting a template DNA with a primer in the presence of sufficient glycerol or ethylene glycol to lower the melting temperature of template DNA and primer hybrids below 70.degree. C. and a DNA polymerase under conditions in which the DNA polymerase can cause primer extensions and is stable to the temperature at which the reaction mixture is heated to denature the primer extension product from the template nucleic acid; and a kit suitable for use in cycle primer extension reaction including the necessary primers, buffers and enzymes required for the procedure, and glycerol.

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patent: 5340714 (1994-08-01), Katsilometes
Smith et al., "Using Cosolvents to Enhance PCR Amplification", Amplifications, A Fosrum for PCR Users, pp. 16-17.
Fuller, 216 Methods in Enzymology 329, 1992, "Modified T7 DNA Polymerase for DNA Sequencing".
Kieleczawa, J. et al., 258 Science 1787, 1992.

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