Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
2007-10-23
2007-10-23
Saidha, Tekchand (Department: 1652)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C435S183000, C435S189000, C435S152000, C435S320100, C435S410000, C435S468000, C536S023200
Reexamination Certificate
active
11244732
ABSTRACT:
This invention provides cyanobacteria as an alternative source of ahas and pds genes for plant transformations and for selectable markers. In particular, it provides for cyanobacteria, for example,Synechocystis, as a source of genes encoding herbicide insensitive proteins, and elements of genes for control of expression in plastids. Nucleic acid fragments, both the acetolactate synthase (ahas) large subunit and the ahas small subunit, were found to provide herbicide resistance. Also, the present invention provides novelSynechocystismutant phytoene desaturase (PDS) gene conferring resistance to 4′-fluoro-6[(alpha,alpha,alpha,-trifluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improvements to method involving cyanobacteria for the screening of compounds, including a new high-through-put protocol that is a rapid and cost effective way to identify target site genes.
REFERENCES:
Windhovel, U., et al., “Engineering Cyanobacterial Models Resistant to Bleaching Herbicides”, Pestic. Biochem. Physiol., 1994, 49: 63-71.
Sandmann, G., et al., “Phytoene Desaturase, the Essential Target for Bleaching Herbicides”, Weed science, 1991, 39:474-479.
Windhovel, U., et al.,“Expression of Erwinia uredovora Phytoene Desaturase in Synechococcus PCC7942 Leading to Resistance against . . . ”, Plant Physiol., 1994, 104:119-125.
Linden,H., et al., “Biochemical Characterization of Synechococcus Mutants Selected against the Bleaching Herbicide Norflurazon”, Pestic.Biochem.Physiol.,1990,36:46-51.
Chamovitz,D., et al., “The molecular basis of resistance to the herbicide norflurazon”, Plant Mol. Biol., 1991, 16:967-974.
Martinez-Ferez,I.M.,et al., “Nucleotide sequence of the phytoene desaturase gene from Synechocystis sp. PCC6803 and characterization . . . ”Plant Mol.Biol.,1992,18:981-983.
Martinez-Ferez,I.,et al.,“Mutagenesis of an Amino Acid Responsible in Phytoene Desaturase from Synechocystis for Binding . . . ”, Pestic.Biochem.Physiol.,1994,48:185-190.
Synechocystis sp. pds gene for phytoene desaturase X62574. Plant Mol. Biol. 18(5), 981-983 (1992).
Kakefuda Genichi
Zhen Rui-Guang
BASF Plant Science GmbH
Ruoying Chen
Saidha Tekchand
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