Cyanobacterial nucleic acid fragments encoding proteins...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase

Reexamination Certificate

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C435S252300, C435S320100, C536S023200, C530S350000, C800S278000, C800S300000

Reexamination Certificate

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07083967

ABSTRACT:
This invention provides an alternative source of ahas and pds nucleic acids for plant transformation and selection. In particular, the invention provides ahas and pds nucleic acids from cyanobacteria, for example,Synechocystis, and expression elements of these genes for control of expression in plastids. The invention further provides nucleic acids encoding the acetolactate synthase (ahas) large subunit and the ahas small subunit which were found to provide herbicide resistance to plants. The present invention also provides a novelSynechocystismutant phytoene desaturase (PDS) gene conferring resistance to 4′-fluoro-6-[(alpha, alpha, alpha,-trfluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improved methods involving cyanobacteria for the screening of compounds, including a new high throughput protocol that is a rapid and cost effective way to identify target site genes.

REFERENCES:
patent: 5378824 (1995-01-01), Bedbrook et al.
patent: 5661017 (1997-08-01), Dunahay et al.
patent: 6343473 (1994-12-01), None
patent: 96/28014 (1996-09-01), None
patent: 98/06862 (1998-02-01), None
patent: 98/20144 (1998-05-01), None
Milano et al. [J. Gen. Microbiol. (1992), 138 : 1399-1408] See the attached sequence search alignment between Applicantions' SEQ ID NO: 6 and Accession No. M75906.
Babczinski et al., 1995 Pestic. Biochem. Physiol.,52(1): 33-44 “Substituted Tetrahydropyrimidinones: A New Herbicidal Class of Compounds Inducing Chlorosis by Inhibition of Phytoene Desaturation”.
Böger, P. and Sandmann, G., 1998 Pesticide Outlook, 6:29-35 “Carotenoid Biosynthesis Inhibitor Herbicides—Mode of Action and Resistance Mechanisms”.
Chamovitz et al., 1993 J. Biol. Chem. 23: 17348-53. vol. 268 “Molecular and Biochemical Characterization of Herbicide-Resistant Mutants of Cyanobacteria Reveals that Phytoene Desaturation is a Rate-Limiting Step in Carotenoid Biosynthesis”.
Clarke et al., 1985 Pestic. Biochem. Physiol., 23(3): 335-340 “Phytotoxicity of m-Phenoxybenzamides: Inhibition of Cell-Free Phytoene Desaturation”.
Duggleby, 1997 Gene, 190: 245-249 “Identification of an Acetolactate Synthase Small Subunit Gene in Two Eukaryotes”.
Dzelskalns & Bogorad, 1988 The EMBO Journal, vol. 7: 333-338 “Molecular Analysis of a Mutant Defective in Photosynthetic Oxygen Evolution and Isolation of a Complementing Clone by a Novel Screening Procedure”.
Freidberg, D. et al., 1990 Z Naturforsch, C, 45(5): 538-543 “Molecular Characterization of Genes Coding for Wild-Type and Sulfonylurea-Resistant Acetolactate Synthase in the CyanobacteriumSynechococcusPCC7942”.
Hattori et al., 1995 Mol. & Gen. Genet., 246: 419-425 “An Acetohydroxy Acid Synthase Mutant Reveals a Single Site Involved in Multiple Herbicide Resistance”.
Kaneko et al., 1995 DNA Research 2:153-166 “Sequence Analysis of the Genome of the Unicellular CyanobacteriumSynechocystissp. Strain PCC6803. I. Sequence Features in the 1 Mb Region from Map Positions 64% to 92% of the Genome”.
Kowalczyk-Schroder & Sandmann, G., 1992 Pestic. Biochem. Physiol., 42(1): 7-12 “Interference of Fluridone with the Desaturation of Phytoene by Membranes of the CyanobacteriumAphanocapsa”.
Linden et al., 1990 Pesticide Biochem. Physiol., 36: 46-51 Biochemical Characterization ofSynechococcusMutants Selected against the Bleaching Herbicide Norflurazon.
Martinez-Ferez et al., 1992 Plant Molecular Biology 18: 981-983 “Nucleotide Sequence of the Phytoene Desaturase Gene fromSynechocystissp. PCCC 6803 and Characterization of a New Mutation which Confers Resistance to the Herbicide Norflurazon”.
Martinez-Ferez et al., 1994 Pestic. Biochem. Physiol., 48: 185-190. “Mutagenesis of an Amino Acid Responsible in Phytoene Desaturase fromSynechocystisfor Binding of the Bleaching Herbicide Norflurazon”.
Miflin, B.J., 1971 Arch Biochem. Biophys., 146: 542-550 “Cooperative Feedback Control of Barley Acetohydroxyacid Synthetase by Leucine, Isoleucine, and Valine”.
Milano et al., 1992 J. Gen Microbiol 138: 1399-1408 “Molecular Characterization of the Genes Encoding Acetohydroxy Acid Synthase in the CyanobacteriumSpirulina platensis”.
Porter, R.D., 1988 “[78] DNA Transformation,” Methods in Enzymology, 167: 703-712.
Powell, et al., 1990 Br. Phycol. J., 25(1): 93 “Antibody probes to Investigate the Outer Surface of the Gas Vesicle”.
Sandmann et al., 1991 Weed Science, 39: 474-479. “Phytoene Desaturase, the Essential Target for Bleaching Herbicides”.
Sandmann et al., 1996 Z Naturforsch, 51(7-8): 534-538 “A New Non-Radioactive Assay of Phytoene Desaturase to Evaluate Bleaching Herbicides”.
Sandmann & Fraser, 1993 Z Naturforsch, C, 48(3-4): 307-311 “Differential Inhibition of Phytoene Desaturases from Diverse Origins and Analysis of Resistant Cyanobacterial Mutants”.
Sandmann et al., 1992 Res. Photosynth. Proc. Int. Congr., 3: 51-54. “Diversity of Phytoene Desaturating Enzymes and Corresponding Genes Involved in Carotenoid Biosynthesis of Photoautotrophic Prokaryotes”.
Sandmann et al., 1992 Pestic. Biochem. Physiol., 42(1): 1-6 (1992) “Quantitative Structure-Activity Relationship of Fluridone Derivatives with Phytoene Desaturase”.
Sandmann, 1993 Target Assays Mod. Herbic. Relat. Phytotoxic Compd., 15-20. “In Vitro Assay System for Phytoene Desaturase Inhibitors with Isolated Thylakoids”.
Singh et al., 1988 Anal. Biochem., 171:173-179. (1988) “Assay of Acetohydroxyacid Synthase”.
Singh et al., 1988 J. Chromatography, 444: 251-261 “Separation and Characterization of Two Forms of Aceto-Hydroxy Acid Synthase from Black Mexican Sweet Corn Cells”.
Weinstock et al., 1992 J. Bacteriology., 174: 5560-5566 “Properties of Subcloned Subunits of Bacterial Acetohydroxy Acid Synthases”.
Williams, 1988, Methods in Enzymology, 167: 766-778 “[85] Construction of Specific Mutations in Photosystem II Photosynthetic Reaction Center by Genetic Engineering Methods inSynechocystis6803”.
Windhöevel et al., 1994 Pestic. Biochem. Physiology, 49(1): 63-71 “Engineering Cyanobacterial Models Resistant to Bleaching Herbicides”.
Windhöevel et al., 1997 Pestic. Biochem. Physiol., 57(1): 68-78 “Genetic Engineering of Resistance to Bleaching Herbicides Affecting Phytoene Desaturase and Lycopene Cyclase in Cyanobacterial Carotenogenesis”.
Windhöevel et al., 1994 Plant Physiology, 104(1): 119-125 “Expression of Erwinia uredovora Phytoene Desaturase inSynechococcusPCC7942 Leading to Resistance against a Bleaching Herbicides”.

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