Organic compounds -- part of the class 532-570 series – Organic compounds – Four or more ring nitrogens in the bicyclo ring system
Patent
1998-04-14
1999-12-14
Richter, Johann
Organic compounds -- part of the class 532-570 series
Organic compounds
Four or more ring nitrogens in the bicyclo ring system
544277, 544317, 546 23, 546 24, 548113, 548547, 548548, 548549, 548579, 548565, 548453, 549473, C07F 909, C07F 9141, C07D207408, C07D48704
Patent
active
060020032
ABSTRACT:
Activating groups for cyanine dyes used to label chain terminators in nucleotide sequencing, based on N-hydroxyphthalimide, are disclosed. From these activating groups, activated dyes of the present Invention are prepared which react with the derivitized nucleotide chain terminators to give a labeled chain terminator of the present Invention. The activating groups of the present Invention allow the dye-chain terminator reaction to occur at a much higher yield and with much greater selectivity for the mono-substituted product, compared with the prior art.
REFERENCES:
Evangelista et al, Characterization of Fluorescent Nucleotide Triphosphates . . . , Analytical Biochemistry, 235, 89-97, Dec. 1996.
Dobashi Thomas S.
Shen Gene G.-Y.
Beckman Instruments Inc.
Harder P. R.
Keating Dominic
May William H.
Richter Johann
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