Cyanide-free reagent and method for the determination of hemoglo

Details Cyanide-free reagent and method for the determination of hemoglo Cyanide-free reagent and method for the determination of hemoglo

1996-10-15

1999-02-02

Wallenhorst, Maureen M.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

436 8, 436 17, 436 63, 436166, 436177, 424 9373, G01N 3372

Patent

active

058664289

ABSTRACT:
A cyanide-free method and reagent for determining the concentration of total hemoglobin in a whole blood sample accurately in less than 10 seconds including a ligand selected from the group consisting of imidiazole, imidazole derivatives, N-hydroxyacetamide, N-hydroxyl amine, pyridine, oxazole, thiazole, pyrazole, pyrimidine, purine, quinoline, and isoquinoline, and a surfactant with strong erythrolytic capability selected from the group consisting of lauryl dimethylamine oxide and octylphenoxy polyethoxyethanol. The reagent pH is adjusted to about 11 to about 14. Rapid mixing of the reagent with a blood sample leads to the formation of a stable chromogen whose absorbance can be measured between 540 and 550 nm. The cyanide-free reagent is ideal for use on an automated high through-put clinical hematology analyzer.

REFERENCES:
patent: 4200435 (1980-04-01), Stroupe et al.
patent: 4255385 (1981-03-01), Stroupe et al.
patent: 4274978 (1981-06-01), Moore
patent: 4853338 (1989-08-01), Benezra et al.
patent: 5242832 (1993-09-01), Sakata
patent: 5506144 (1996-04-01), Sundrehagen
R. Zander, et al., "Alkaline haematin D-575, a new tool for the determination of haemoglobin as an alternative to the cyanhaemiglobin method. I. Description of the method", Elsevier Science Publishers, 83-93 (1984).
M.J. Malin, et al, "Mechanism of automated alkaline methods for the determination of hemoglobin in whole blood based on the micellization of ligated heme in the presence and absence of cyanide", Analytica Chemica Acta, 262 (1992) 67-77.

Affiliated with

Also associated with

Rating

Cyanide-free reagent and method for the determination of hemoglo does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cyanide-free reagent and method for the determination of hemoglo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cyanide-free reagent and method for the determination of hemoglo will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1116736