Cyanide-free reagent and method for hemoglobin determination and

Details Cyanide-free reagent and method for hemoglobin determination and Cyanide-free reagent and method for hemoglobin determination and

1994-12-23

1998-11-10

Achutamurthy, Ponnathapura

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

436 8, 436 10, 436 17, 436 18, 436 63, 252397, 2524081, G01N 3372, G01N 3100, C09K 1500

Patent

active

058343156

ABSTRACT:
A reagent composition which does not contain cyanide ions and a method for measuring hemoglobin concentration in a blood sample. In addition, the reagent composition can be used to measure hemoglobin concentration and differentiate at least two subpopulations of leukocytes from the same reaction product of the reagent composition and a blood sample. The reagent composition comprises at least one lysing agent selected from the group consisting of a quaternary ammonium salt, a pyridinium salt, and combinations thereof, in an amount effective to adequately lyse the erythrocytes and elute the hemoglobin, an antioxidant in an amount effective to convert the released hemoglobin into to a hemochromogen. The pH can be adjusting with a pH agent to provide a pH ranging from 5 to 11.5.

REFERENCES:
patent: 3874852 (1975-04-01), Hamill
patent: 3962125 (1976-06-01), Armstrong
patent: 4185964 (1980-01-01), Lancaster
patent: 4200435 (1980-04-01), Stroupe et al.
patent: 4255385 (1981-03-01), Stroupe et al.
patent: 4346018 (1982-08-01), Carter et al.
patent: 4485175 (1984-11-01), Ledis et al.
patent: 4521518 (1985-06-01), Carter et al.
patent: 4528274 (1985-07-01), Carter et al.
patent: 4617275 (1986-10-01), Matsuda et al.
patent: 4656139 (1987-04-01), Matsuda et al.
patent: 4745071 (1988-05-01), Lapioca et al.
patent: 4800167 (1989-01-01), Bailey et al.
patent: 4853338 (1989-08-01), Benezra et al.
patent: 4968629 (1990-11-01), Lapioca et al.
patent: 5242832 (1993-09-01), Sakata
patent: 5250437 (1993-10-01), Toda et al.
patent: 5686308 (1997-11-01), Young et al.
Malin, Michael J., et al., "Evaluation of 24-Second Cuanide-Containing and Cyanide-Free Methods for Whole Blood Hemoglobin on the Technicon H*1 Analyzer with Normal and Abnormal Blood Samples," AJCP, Sep. 1989 pp. 286-294.

Affiliated with

Also associated with

Rating

Cyanide-free reagent and method for hemoglobin determination and does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cyanide-free reagent and method for hemoglobin determination and, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cyanide-free reagent and method for hemoglobin determination and will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1515927