Chemistry: analytical and immunological testing – Hemoglobin – myoglobin – or occult blood
Patent
1994-12-23
1998-11-10
Achutamurthy, Ponnathapura
Chemistry: analytical and immunological testing
Hemoglobin, myoglobin, or occult blood
436 8, 436 10, 436 17, 436 18, 436 63, 252397, 2524081, G01N 3372, G01N 3100, C09K 1500
Patent
active
058343156
ABSTRACT:
A reagent composition which does not contain cyanide ions and a method for measuring hemoglobin concentration in a blood sample. In addition, the reagent composition can be used to measure hemoglobin concentration and differentiate at least two subpopulations of leukocytes from the same reaction product of the reagent composition and a blood sample. The reagent composition comprises at least one lysing agent selected from the group consisting of a quaternary ammonium salt, a pyridinium salt, and combinations thereof, in an amount effective to adequately lyse the erythrocytes and elute the hemoglobin, an antioxidant in an amount effective to convert the released hemoglobin into to a hemochromogen. The pH can be adjusting with a pH agent to provide a pH ranging from 5 to 11.5.
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Riesgo Mirta I.
Young Carole Jo
Achutamurthy Ponnathapura
Alter Mitchell E.
Coulter Corporation
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