Cyanide-free hemoglobin reagent

Details Cyanide-free hemoglobin reagent Cyanide-free hemoglobin reagent

1988-12-13

1989-08-01

Richman, Barry S.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

356 40, 2504612, 436 15, 436 63, G01P 3348

Patent

active

048533386

ABSTRACT:
A method and reagent useful therein are cyanide-free and capable of providing a rapid indication, e.g., less than 30 seconds, of total hemoglobin in a blood sample, so as to fine particular application in automated hematological instrumentation. The novel reagent comprises an ionic surfactant, a pH of at least about 11.3 and is free of ionic cyanide. The ionic surfactant itself can impart the required pH or a strong base independent of such surfactant may be included.

REFERENCES:
patent: 3874852 (1975-04-01), Hamill
patent: 4185964 (1980-01-01), Lancaster
patent: 4286963 (1981-09-01), Ledis et al.
Oshiro et al., Clin. Biochem. 15 83 (1982).
Zander et al., Clin. Chem. Acta. 136, pp. 83-93 (1984).

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