Cup-shaped vertical slab gel electrophoresis apparatus

Details Cup-shaped vertical slab gel electrophoresis apparatus Cup-shaped vertical slab gel electrophoresis apparatus

1997-03-14

1999-08-17

Warden, Robert

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

G01N 2726

Patent

active

059389095

ABSTRACT:
The titled apparatus mainly comprises an outer casting member having a hollow frustum channel lined detachably by a flexible sheet, an inner casting member having an exterior lateral wall of frustum shape being placed within the sheet-lined channel of the outer casting member, a gel slab sandwiched concentrically in the space between the interior wall of the sheet-lined channel and the exterior lateral wall of the inner casting member, and a method to expose the entire gel by first slipping off the outer casting member from the flexible sheet, then peeling off the flexible sheet from the gel slab, and then taking the entire gel slab off from the inner casting member. The apparatus provides a vertical electrophoresis means for both agarose gel electrophoresis and polyacrylamide gel electrophoresis with the advantages of large sample capacity, higher sensitivity, short analysis time, and simple operation.

REFERENCES:
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patent: 4729823 (1988-03-01), Guevara, Jr.
patent: 5525202 (1996-06-01), Evans et al.
J. Hejgaard, Cylindrical Slab Gel Electrophoresis in a Single Tank. Analytical Biochemistry vol. 90, 832-834 (1978).
Junichi Akiyama, Cylindrical Stationary Phase Electrophoresis Apparatus. Chemical Abstract vol. 110, 72141j (1989).
William C. Black IV, et al., Vertical Slab Gel Electrophoresis on a large Number of Samples. Analytical Biochemistry vol.138 210-216 (1984).
Kazuo Yoshida, A Highly Simplified Horizontal Electrophoretic Apparatus . . . Analytical Biochemistry, vol. 130, 246-259 (1983).
Branko Kozulic, Looking at Bands from Another Side. Analytical Biochemistry vol. 216, 253-261 (1994).
Michael Albin, et al., Sensitivity Enhancement for Capillary Electrophoresis. Analytical Chemistry vol. 65, 489A-497A (1993).

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