Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1989-11-29
1992-11-24
Schain, Howard E.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435286, 435311, 4352401, C12N 502, C12N 500, C12M 302, C12M 306
Patent
active
051660679
ABSTRACT:
A culturing method and system are disclosed in which a cell culture broth in withdrawn from a culturing apparatus to a filteration apparatus. The withdrawn broth in brought into contact with a membrane for precision filteration. Then a non-toxic gas is brown into the broth, while filtering the broth to separate the cells and breaking foams occurring on the surface of the broth due to gas bubbling. The separated cells are returned to the vessel and the filterate is withdrawn from the filteration apparatus.
The method and system have such advantages that a perfusion culture of a long period of time can be realized because the clogging of the filteration membrane is effectively prevented.
REFERENCES:
patent: 3898045 (1975-08-01), Bowley
patent: 4177138 (1979-12-01), Mastuzaki et al.
patent: 4335215 (1982-06-01), Tolbert et al.
patent: 4426450 (1984-01-01), Donofrio
patent: 4775471 (1988-10-01), Nagai et al.
patent: 4833078 (1989-05-01), Hsieh
patent: 4900669 (1990-02-01), Hatch et al.
"The Large-Scale Cultivation of Mammalian Cells", Joseph Feder et al., Scientific American, Jan., 1983.
Haga Ryoichi
Ishida Masahiko
Matsuzaki Harumi
Elliott George C.
Hitachi , Ltd.
Schain Howard E.
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