Culturing method of mesenchymal stem cell

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology

Reexamination Certificate

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C435S373000, C435S325000, C435S347000, C435S352000, C435S363000, C435S366000, C435S372000, C435S377000, C435S395000

Reexamination Certificate

active

08039256

ABSTRACT:
To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 at 37° C., and making a culture at a temperature 37±2° C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring.

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