Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1996-12-12
1999-02-23
Naff, David M.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
435384, 435387, 435395, 435404, 435405, 435406, C12N 508, C12N 506, C12N 1100
Patent
active
058743065
ABSTRACT:
Human pancreatic endocrine cells are proliferated without loss of hormone function in a culture medium containing extracellular matrix from bladder carcinoma cell lines in the substantial absence of hepatocyte growth factor/scatter factor. Proliferation is preferably carried out in the substantial absence of any peptide growth factors and nicotinamide. The cells may be proliferated in a monolayer on a solid substrate. Islets and islet-like cell clusters are proliferated without loss of insulin-secreting function by incubation in a medium containing extracellular matrix from a human bladder carcinoma cell line, preferably cell line ATCC HTB-9.
REFERENCES:
patent: 5315000 (1994-05-01), Degen
patent: 5464815 (1995-11-01), Chamow et al.
patent: 5510263 (1996-04-01), Quaranta et al.
patent: 5532341 (1996-07-01), Welte et al.
patent: 5571509 (1996-11-01), Comoglio et al.
patent: 5587309 (1996-12-01), Rubin et al.
A. Hayek, et al., "Growth Factor/Matrix-Induced Proliferation of Human Adult .beta.-Cells," Diabetes (Dec. 1995) 44: 1458-1460.
G.M. Beattie, et al., "Regulation of Proliferation and Differentiation of Human Fetal Pancreatic Islet Cells by Extracellular Matrix, Hepatocyte Growth Factor, and Cell-Cell Contact," Diabetes (Sep. 1996) 45: 1223-1228.
G.M. Beattie, et al., "Acid .beta.-Galactosidase: A Developmentally Regulated Marker of Endocrine Cell Precursors n the Human Fetal Pancreas," Journal of Clinical Endocrinology and Metabolism (1994) 78(5): 1232-1240.
G.M. Beattie, et al., "Functional Impact of Attachment and Purification in the Short Term Culture of Human Pancreatic Islets," Journal of Clinical Endocinology and Metabolism (1991) 73(1): 93-98.
Beattie Gillian M.
Hayek Alberto
Naff David M.
The Regents of the University of California
LandOfFree
Culturing human pancreatic endocrine cells in medium containing does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Culturing human pancreatic endocrine cells in medium containing , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Culturing human pancreatic endocrine cells in medium containing will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-306202