Cultures of yeast of the genus Pichia altered by site selective

Details Cultures of yeast of the genus Pichia altered by site selective Cultures of yeast of the genus Pichia altered by site selective

1989-09-01

1992-08-04

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

C12N 119

Patent

active

051358689

ABSTRACT:
Method for the site specific genomic modification of yeasts of the genus Pichia and novel DNA sequences useful therefore are provided. Pichia is transformed with a serially arranged linear DNA fragment comprising first and second insertable DNA fragments, which flank a marker gene. The insertable DNA sequences are homologous to defined portions of the Pichia genome and integrate by recombination at such defined sites. The resulting transformed organism contains the marker gene and any additional DNA sequences which are positioned between the insertable DNA fragments. In addition, the resulting transformed organism has either a disrupted or deleted sequence at the site of the genomic modification. Enhanced production of heterologous gene products is observed when using as the host for expression strains in which the alcohol oxidase gene has been disrupted. Upon disruption of the primary alcohol oxidase gene of Pichia, the existence of a second alcohol oxidase gene in Pichia was also discovered.

REFERENCES:
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patent: 4530901 (1985-07-01), Weissmann
patent: 4895800 (1990-01-01), Tschopp et al.
Proc. Natl. Acad. Sci. USA, vol. 78 (10), T. Orr-Weaver et al, pp. 6354-6358 Oct. 1981.
Cell, vol. 33, J. Szostak et al, pp. 25-35 May 1983.
Journal of Bacteriology, vol. 161 (1), H. Boucherie, pp. 385-392 Jan. 1985.

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