Cultures of GFAP+ nestin+ cells that differentiate to neurons

Details Cultures of GFAP+ nestin+ cells that differentiate to neurons Cultures of GFAP+ nestin+ cells that differentiate to neurons

2005-04-12

2005-04-12

Kemmerer, Elizabeth (Department: 1647)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S366000, C435S368000, C435S378000

Reexamination Certificate

active

06878543

ABSTRACT:
Cultures of cells immunoreactive for glial fibrillary acidic protein (GFAP), as well as for the intermediate filament marker nestin were grown in a medium including epidermal growth factor (EGF) and serum. The cultured cells had the morphology of astroglial cells. The cells can be proliferated in adherent or suspension cultures. Depending on the culture conditions, the cells can be induced to differentiate to neurons or glial cells. The cultures can be expanded over a large number of passages during several months, and survive, express an astroglial phenotype and integrate well after transplantation into both neonatal and adult rat forebrain.

REFERENCES:
patent: 4352883 (1982-10-01), Lim
patent: 4353888 (1982-10-01), Sefton
patent: 4968733 (1990-11-01), Muller et al.
patent: 4976859 (1990-12-01), Wechs
patent: 5071741 (1991-12-01), Brockbank
patent: 5084350 (1992-01-01), Chang et al.
patent: 5158881 (1992-10-01), Aebischer et al.
patent: 5284761 (1994-02-01), Aebischer et al.
patent: 5338839 (1994-08-01), McKay et al.
patent: 5750376 (1998-05-01), Weiss et al.
patent: 5753506 (1998-05-01), Johe
patent: 5766948 (1998-06-01), Gage et al.
patent: 5800828 (1998-09-01), Dionne et al.
patent: 5851832 (1998-12-01), Weiss et al.
patent: 5968829 (1999-10-01), Carpenter
patent: 6040180 (2000-03-01), Johe
patent: 6258353 (2001-07-01), Isacson et al.
patent: 6294383 (2001-09-01), Isacson et al.
patent: 6497872 (2002-12-01), Weiss et al.
patent: 20020151066 (2002-10-01), Rubenstein et al.
patent: 20030032118 (2003-02-01), Weiss et al.
patent: WO 9204033 (1992-03-01), None
patent: WO 9219195 (1992-11-01), None
patent: WO 9314191 (1993-07-01), None
patent: WO 9505452 (1995-02-01), None
patent: WO 9507611 (1995-03-01), None
patent: WO 9604368 (1996-02-01), None
patent: WO 9609543 (1996-03-01), None
patent: WO 9627287 (1996-09-01), None
patent: WO 9629862 (1996-10-01), None
patent: WO 9814058 (1998-04-01), None
patent: WO 9830678 (1998-07-01), None
patent: WO 0024897 (2000-05-01), None
Daadi and Weiss (Jun. 1, 1999) “Generation of Tyrosine Hydroxylase-Producing Neurons from Precursors of the Embryonic and Adult Forebrain.” The Journal of Neuroscience 19(11): 4484-4497.*
Kalyani et al. (Oct. 1, 1998) “Spinal Cord Neuronal Precursors Generate Multiple Neuronal Phenotypes in Culture.” The Journal of Neuroscience 18(19): 7856-7868.*
Santa-Olalla et al. (1995) Epidermal Growth Factor, Transforming Growth Factor-alpha, and Basic Fibroblast Growth Factor Differentially Influence Neural Precursor Cells of Mouse Embryonic Mesencephalon. J. Neurosci Res 42(2): 172-83.*
Rutka et al. (1999) Co-Expression of Nestin and Vimentin Intermediate Filaments in Invasive Human Astrocytoma Cells. Int. J. Devel. Neuroscience 17(5-6): 503-515.*
Hulspas et al. (Nov. 1997) “In Vitro Cell Density-Dependent Clonal Growth of EGF-Responsive Murine Neural Progenitor Cells under Serum-Free Conditions.” Experimental Neurology 148(1): 147-156.*
Miyaguchi (Oct. 1997) “Ultrastructure of Intermediate Filaments of Nestin- and Vimentin-Immunoreactive Astrocytes in Organotypic Slice Cultures of Hippocampus.” Journal of Structural Biology 120(1): 61-68.*
Schinstine & Iacovitti (Sep. 1996) “Expression of Neuronal Antigens by Astrocytes Derived from EGF-Generated Neuroprogenitor Cells.” Experimental Neurology 141(1): 67-78.*
Jessen et al., 13 Neurocytology 923-034 (1984).
Fields et al., 8. J. Neuroimmunol.311-330 (1989).
Lendahl et al., 60 Cell 585-595 (1990).
Gallo et al., 15 J. Neurosci 394-406 (1995).
Lin et al., 2 Neurobiol. Dis. 79-85 (1995).
Docisch et al., 97 Cell 703-16 (1999).
Johansson et al, 96 Cell 25-34 (1999).
Lagenaur & Schachner, 15 J. Supranol. Struct Cell Biochem. 335-346(1981).
Lagenaur et al., 23 J. Neurobiol. 71-88 (1992).
Zhou & Lund. 387 J. Comp.Neurol. 145-55 (1992).
Mi et al., 106 Dev. Brain Res. 145-54 (1998).
Hankin & Lund. 263 J. Comp. Neuro. 455-466 (1987).
Wictorin et al., 3 Eur. J. Neurosci. 86-101 (1991).
Hunter et al., 33. J. Neurobiol 459-472 (1997).
Mission et al., 44 Dev. Brain Res. 95-108 (1988).
Weiss et al., 19 Trends Neurosci. 387-93 (1996).
Reynolds et al., 12 J. Neurosci. 4565-74 (1992).
Reynolds & Weiss, 255 Science 1707-10 (1992).
Reynolds & Weiss, 175 Dev. Biol. 1-13 (1996).
Hammang et al., 147 Exp. Neurol. 84-95 (1997).
Svendsen et al., 148 Exp. Neurol. 135-46 (1997).
Vescovi et al., 156 Exp. Neurol. 71-83 (1999).
Karlson et al., 65 Biophysical J. 2524-2536 (1993).
Bjorklund, 14 Trends Neurosci. 319-322 (1991).
Gruber, 54 Transplantation 1-11 (1992).
Zheng et al., 88 Proc. Natl. Acad. Sci. 8067-8071 (1991).
Duncan et al, 17 J. Neurocytology 351-360 (1988).
Lundberg et al., 139 Exp. Neurol. 39-53 (1996).
Tornatore et al., 5 Cell Transplant 145-163 (1996).
Ridet et al., 10 Hum. Gene Ther. 271-280 (1999).
Faaland et al., II Mol. Cell Biol. 2697-2703 (1991).
Weiss et al., 16 J. Neurosci. 7599-609 (1996).
Cunningham & McKay, 47 J. Neurosci. Methods 105-14 (1993).
Brustle et al., 15 Neuron 1275-85 (1995).
Olsson et al., 79 Neuroscience 57-78 (1997).
Gales et al., 84 Neuroscience 1013-1023 (1998).
Lin, Q. et al., 8 Human Gene Therapy 331-339 (1997).
Yong, V.W. et al., 88 Proc. Nat. Aca. Sci. USA 7016-7020 (1991).
Palmer, T.D. et al., 8 Mol. and Cell. Neur. 389-404 (1997).
Eriksson, Cecilia et al. 164 Exp. Neur. 184-199 (2000).
Eriksson, C. et al., 12 Supp. 11 Eur. J. of Neur. 263 (2000).
Fagerstrom, C. et al., 12 Supp. 11 Eur. J. of Neur. 326 (2000).

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