Cultured Xenopus laevis cell lines expressing mutant...

Details Cultured Xenopus laevis cell lines expressing mutant... Cultured Xenopus laevis cell lines expressing mutant...

2003-08-19

2008-05-13

Spector, Lori (Department: 1647)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C435S320100, C435S325000

Reexamination Certificate

active

07371843

ABSTRACT:
Using protein visualization techniques, cell lines stably expressing GFP-fused full-length and mutant APCs were obtained from culturedXenopus laevisrenal epithelial A6 cells. The use of these cells showed that mutant APCs, whose C-terminal region is absent, induced piling up of cells. Furthermore, piled up cells from mutant APC-expressing cell lines were proven to maintain the intercellular adhesive structure, representing a phenomenon similar to polyp formation in individual organisms (mice).

REFERENCES:
patent: WO 01/16167 (2001-03-01), None
patent: WO 01/16167 (2001-03-01), None
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