Cultured muscle cells with high metabolic activity and...

Details Cultured muscle cells with high metabolic activity and... Cultured muscle cells with high metabolic activity and...

2006-09-21

2010-11-09

Afremova, Vera (Department: 1657)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

C435S375000, C435S366000

Reexamination Certificate

active

07829334

ABSTRACT:
The object of the present invention is to provide a method of preparing excellent cultured muscle cells having high metabolic capacity and insulin responsiveness, and further provide a method for the measurement of sensitive metabolic capacity using the cells. The present invention is a method of preparing myotube cells, comprising a step (1) of culturing myoblast cells, a step (2) of differentiation-inducing the myotube cells into the myoblast cells in a culture medium with a high content of amino acids, and a step (3) of applying an electric pulse to the differentiation-induced myotube cells.

REFERENCES:
patent: 2005-27501 (2005-02-01), None
Thelen et al. “Electrical stimulation of C2C12 myotubes induces contractions and represses thyroid-hormone-dependent transcription of the fast-type sarcoplasmic-reticulum Ca2+—ATPase gene”. Biochem J. (1997), 321: 845-848.

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