Cultured cell line that inducibly expresses the hepatitis B viru

Details Cultured cell line that inducibly expresses the hepatitis B viru Cultured cell line that inducibly expresses the hepatitis B viru

1995-06-05

1998-03-03

Woodward, Michael P.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 5, 435 71, 435 59, 435 701, 435325, C12N 1551, C12N 510, C12Q 170

Patent

active

057233195

ABSTRACT:
Novel cell lines and methods are provided for inducibly expressing the Hepatitis B virus (HBV) genome within cultured cells. Cells are stably transformed with an HBV genome under control of an operator/promoter target sequence which can activate expression of the HBV genome when bound by a cognate transactivator protein. The cell lines are also stably transformed with a gene that encodes and expresses a suitable transactivator protein. Cells may be maintained under non-inducing conditions whereby the transactivator protein is unable to interact with the operator/promoter target sequence, so the HBV genome is not expressed. Under appropriate inducing conditions, the transactivator protein is enabled to interact with the operator/promoter target sequence, thereby activating expression of the HBV genome. The inducible system provides an effective and rapid cell-based assay to screen compounds for anti-hepadnaviral activity.

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