Cultured cell construct containing spheroids of cultured...

Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing

Reexamination Certificate

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C435S325000, C435S347000, C435S373000

Reexamination Certificate

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07470424

ABSTRACT:
Parenchymal cells are cultivated on cultivated endothelial cells or cultivated fibroblasts which have been separated by a surface of a specific hydrophilic polymer, and which have been patterned. A culture which contains thus formed patterned spheroids of cultivated parenchymal cells is thereby provided by this invention. This culture maintains a function which is specific to the parenchymal cells over a long period of time.

REFERENCES:
patent: 5976826 (1999-11-01), Singhvi et al.
patent: 6132979 (2000-10-01), Murakami
van Kooten et al., “Influence of substratum wettability on the strength of adhesion of human fibroblasts”, Biomaterials, 1992, 13(13):897-904.
Takezawa et al., Journal of Cell Science, 101, 495-501, 1992.

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