Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1994-09-02
1997-11-11
Tran, Lien
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
4352866, 4352891, 422102, C12M 102
Patent
active
056863012
ABSTRACT:
A cell culture vessel which permits gentle but vigorous intermixing and suspending of the cell culture during rotation. The culture vessel features a cell mixing and suspending device combined with a gas exchange membrane such that ample gas exchange occurs without the need to feed oxygen to the cells. The cell mixing and suspending device comprises a baffle system having at least one, and preferably more than one, mixing elements integral with the gas exchange membrane. Movement of the culture vessel, such as for example by rolling, shaking or swirling, moves the mixing elements such that the cells which are to be cultivated are gently intermixed and stably suspended within the cell culture chamber. Pressure variations within the cell culture vessel are accommodated by pressure equalizing device. The pressure equalizing device preferably comprises pressure sheathing made of gas permeable material positioned to form at least a portion of the outer wall of the supply chamber. The pressure sheathing compresses to accommodate increased pressure within the supply chamber. Moreover, the gas permeable material permits additional gas exchange to occur through the supply chamber. Mixing of the nutrient medium is accomplished during movement of the cell culture vessel by way of the extending pressure finger.
REFERENCES:
patent: 3223595 (1965-12-01), Brewer
patent: 4276384 (1981-06-01), Mueller
patent: 4661455 (1987-04-01), Hubbard
patent: 4661458 (1987-04-01), Berry et al.
patent: 4717668 (1988-01-01), Keilman et al.
patent: 4720462 (1988-01-01), Rosenson
patent: 4734372 (1988-03-01), Rotman
patent: 4748124 (1988-05-01), Vogler
patent: 4839046 (1989-06-01), Chandler
patent: 4959322 (1990-09-01), Sakai
patent: 4978616 (1990-12-01), Dean, Jr. et al.
patent: 4988623 (1991-01-01), Schwarz et al.
patent: 5026650 (1991-06-01), Schwarz et al.
patent: 5110741 (1992-05-01), Ohi et al.
patent: 5153131 (1992-10-01), Wolf et al.
patent: 5155034 (1992-10-01), Wolf et al.
patent: 5155035 (1992-10-01), Schwarz et al.
patent: 5288631 (1994-02-01), Baumgartner et al.
patent: 5376548 (1994-12-01), Matsuo et al.
patent: 5449617 (1995-09-01), Falkenberg et al.
Rotary Cell Culture News, Issue No. 1, Apr. 1994, pp. 1-4.
Synthecon, Inc. 1994 Catalog of Products.
NASA Tech Briefs, Inventors of the Year, Johnson Team's Biotech Breakthrough, Mar. 1992, vol. 16, No. 3.
Medical Research System, pp. 56-57, Spinoff, 1993, NASA Publication.
Lewis, Marian L. et al., The Rotary Cell Culture System, A New Dimension for Tissue Culture, Journal of Cellular Biochemistry 51, pp. 265-273 (1993).
Spaulding, et al., Viewpoint, Advances in Cellular Construction, Journal of Cellular Biochemistry, (Mar. 1993), vol. 51, pp. 249-251.
Stephens, T. NASA Allies Tout Biomedical Spinoffs, The Journal of NIH Research, (Dec. 1991) vol. 3, pp. 28-31.
Beardsley, T., Shear Bliss, A Bioreactor Grows Cells That Resemble Real Tissue, Scientific American, (Feb. 1992) p. 27.
Weichert, et. al, In Vitro Production of Monoclonal Antibodies in High Concentration in a New and Easy to Handle Modular Minifermenter, Proceedings of the "World Congress on Alternativess and Animal Use in the life Sciences", Nov. 14-19, 1993, Baltimore, MD/USA.
Falkenberg, et. al, A Simple and Inexpensive High Density Dialysis Tubing Cell Culture System for the In Vitro Production of Monoclonal Antibodies in High Concentration, Journal of Immunological Methods, 165 (1993) 193-206.
1992 Fisher Product Catalog.
Falkenberg Frank W.
Kohn Heinz-Gerhard
Nagels Hans-Otto
Heraeus Instruments GmbH
Tran Lien
LandOfFree
Culture vessel for cell cultures does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Culture vessel for cell cultures, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Culture vessel for cell cultures will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1228173