Culture of totipotent embryonic inner cells mass cells and produ

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal

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800 15, 800 17, 800 24, 435373, 435325, 435378, 435379, 435380, 435381, 435455, 435449, 435450, 435375, 435377, 435383, 435392, C12N 1509, C12N 1500, C12N 500, C12N 1563

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061075434

ABSTRACT:
The use of totipotent embryonic stem cells to provide substantially identical cells for embryo cloning techniques is described. The method includes the culture of loose suspensions of inner cell mass cells of bovine animals to retrieve large populations of stem cells. The invention also describes the use of stem cells in various genetic manipulation techniques.

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