Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Solid support and method of culturing cells on said solid...
Patent
1996-06-19
1997-12-30
Elliott, George C.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Solid support and method of culturing cells on said solid...
435395, C12N 500
Patent
active
057029490
ABSTRACT:
Anchorage-dependent cells are grown on a novel substratum which is believed to increase the oxygenation of the cells and reduce the formation of free-radicals. The substratum consists of a perfluorocarbon reservoir which is coated with a layer of protein (e.g.,collagen or gelatin) which has been perfluoroalkylated. The perfluoroalkylated protein forms a very stable surface to which anchorage-dependent cells can attach and grow. Using this system, mammalian cell cultures have been grown to densities exceeding 10.sup.7 cells/cm.sup.2. The increased density is attributed to the formation of multiple cell layers which resemble tissue-like structures.
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Rappaport Catherine
Trujillo Edward M.
Elliott George C.
Railey II Johnny F.
University of Utah Research Foundation
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