Culture medium supplying method and culture system

Details Culture medium supplying method and culture system Culture medium supplying method and culture system

1991-12-20

1994-05-31

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Condition responsive control process

4352401, 435284, 435289, 435313, C12Q 300, C12M 300

Patent

active

053169050

ABSTRACT:
A culture system comprising at least first and second culture medium tanks including a gas phase, a culture medium phase, at least one gas phase port, and at least one culture medium port. At least one culture vessel. First switching-over members have a plurality of culture medium flow passages connected between the tanks and the culture vessels. The culture medium phase of the first tank flows through the passages to the culture medium phase of the second tank via the culture vessel. Switching-over of the culture medium flow passages causes the culture medium phase of the second tank to flow through the passages to the culture medium phase of the first tank via the culture vessel. Gas supply section comprises at least two pressure chambers and at least two gas ports connected to the pressure chambers. Second switching-over members have formed therein a plurality of gas flow passages allowing a gas to flow from the gas control section to the gas phase in the first and second tanks. Switching-over of the gas flow passages causes the gas flow to switch between the tanks. The gas control section includes gas pressure regulating members, gas flow rate regulating members and gas component regulating members. Gas source switching-over members are included.

REFERENCES:
patent: 4804628 (1989-02-01), Cracauer et al.
patent: 4889812 (1989-12-01), Guinn et al.
patent: 4973558 (1990-11-01), Wilson et al.

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