Culture medium for the detection of E. coli and process for its

Details Culture medium for the detection of E. coli and process for its Culture medium for the detection of E. coli and process for its

1996-08-22

1998-12-08

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 14, 435849, 435 4, C12Q 104, C12Q 102, C12Q 154, C12N 100

Patent

active

058467610

ABSTRACT:
A culture medium for the detection of E. coli and a process for the detection of E. coli utilizing the culture medium are disclosed. The culture medium includes a known culture medium for E. coli, a chromogenic compound derived from indolyl-glucuronic acid derivatives or salts thereof and a non-chromogenic alkyl, alkenyl, or aryl glucuronic acid derivative or salt thereof. The indolyl-glucuronic acid derivative or salts thereof serve as substrates for the beta-D-glucuronidase (GUS) enzyme that by cleavage of the substrate, gives a colored or flourescent derivative. The indolyl-glucuronic acid derivative in the culture medium is between 1:1 and 1:19, and is preferably 1:1 to 1:3. The culture medium may further contain a phosphate at a concentration greater than one gram per liter, and preferably between four and ten grams per liter. The process relates to inoculating the culture medium with a sample or an inoculum obtained from the sample and monitoring the medium for the presence of E. coli.

REFERENCES:
patent: 4923804 (1990-05-01), Ley et al.
patent: 5210022 (1993-05-01), Roth et al.
Ogden et al, "Letters in Applied Microb," vol. 13, pp. 212-215, 1991. Month not available.
Jefferson et al, Pro Natl Acad. Sci., vol. 83, pp. 8447-8451, Nov. 1986.
Letters in Applied Microbiology, vol. 13, No. 4, Oct. 1, 1991, Blackpool UK, pp. 212-215.
Zentralblatt fur Hygiene und Umweltmedizin, vol. 189, No. 3, 1989, Stuttgart Brd, pp. 225-234 month not available.

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