Culture medium for gingival fibroblasts

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Culture medium – per se

Reexamination Certificate

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C435S404000, C435S325000, C435S357000, C435S366000

Reexamination Certificate

active

07951593

ABSTRACT:
The present invention relates to a gingival fibroblast culture medium free of animal serum, comprising an animal cell culture medium, free of animal serum, to which is added:from 0.1 ng/ml to 100 ng/ml bFGF, and/orfrom 1 μg/ml to 50 μg/ml insulin.

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Marcopoulou et al., J Int Acad Periodontol. Jul. 2003;5(3):63-70; Abstract only.
Soory et al, “Anabolic potential of fibroblasts from chronically inflamed gingivae grown in a hyperglycemic cluture medium in the presence or absence of insulin and nicotine.”, Dec. 2003, pp. 1771-1777, vol. 74, No. 12, Journal of Periodontology.
Ta et al, “Heparan sulfate interacting protein (HIP/L29) negatively regulates growth responses to basic fibroblast growth factor in gingival fibroblasts.”, Apr. 2002, pp. 247-252, vol. 81, No. 4. , Journal of Dental Research.
Doucet et al, “Platelet lysates promote mesenchymal stem cell expansion: A safety substitute for animal serum in cell-based therapy applications”, Nov. 1, 2005, pp. 228-236, vol. 205, No. 2., Journal of Cellular Physiology, Liss, New York, NY, USA.
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