Culture medium containing human albumin, process for the prepara

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352402, 4352403, C12N 500

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active

050213490

ABSTRACT:
The present invention relates to a lymphoblastoid cell culture medium making it possible in particular to prepare products which can be administered to man by intravenous injection, the said culture medium containing, in addition to the constituent components of ISCOVE's medium, only human albumin as protein.

REFERENCES:
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patent: 4681848 (1987-07-01), Tsukamoto et al.
Kawamoto et al., 1983, Analytical Biochemistry 130, pp. 445-453 "Development of a Serum Free Medium for Growth of NS1 Mouse Myeloma Cells and Its Application to the Isolation of NS1 Hybridomas".
Iscove et al., "Complete Replacement of Serum by Albumin, Transferrin, and Soybean Lipid in Cultures of Lipopolysaccharide-Reactive B Lymphocytes", J. Exp. Med. vol. 147, 923-933, 1978.
Chemical Abstracts, vol. 101, No. 25, Dec. 17, 1984, p. 399, "Culture of Human Lymphocytes in Serum-Free Medium".
Pharmacia Fine Chemicals, Presentations from Biotech '83, May 4-6, 1983, pp. 39-46, "Purification of Monoclonal Antibodies and Their Use in the Isolation of Biological Products".
Proceedings of the National Academy of Science, U.S.A., vol. 53, 1965 pp. 288-293, "Clonal Growth of Mammalian Cells in a Chemically Defined, Synthetic Medium."

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