Culture medium

Details Culture medium Culture medium

1982-05-18

1985-08-06

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435948, C12N 500, C12R 191

Patent

active

045336377

ABSTRACT:
On the new findings that cyclodextrin shows no cytotoxicity or only slight cytotoxicity on cell growth and that lipophilic substances such as unsaturated fatty acids and lipophilic vitamins when present together with, or included in, cyclodextrin show such effects as cell growth promoting effect and accelerating effect of the productivity of valuable products, a serum-free or serum-reduced culture medium or a substitute composition for serum for a culture medium comprising a cyclodextrin and at least one lipophilic nutrient substance, said cyclodextrin and said lipophilic nutrient substance being preferably in the form of inclusion complex between them, is provided.

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patent: 4198479 (1980-04-01), Tytell et al.
patent: 4367072 (1983-01-01), Vogtle et al.
patent: 4371673 (1983-02-01), Pitha
Yamane et al., "A-Cyclodextrin: A Partial Substitute for Bovine Serum Albumin in Serum Free Culture of Mammalian Cells" Cold Spring Harbor Conferences on Cell Proliferation, vol. 9, (1981), pp. 87-92.
Yamane et al., "A-Cyclodextrin, A Novel Substitute for Bovine Albumin in Serum Free Culture of Mammalian Cells" Proc. Jpn. Acad. Ser. B., (1981), 57(10), pp. 385-389, Chem. Abst., 96:100488r.
Yamane et al., "Role of Bovine Albumin in Serum Free Suspension Cell Culture Medium", Proc. Soc. Exp. Biol. Med., 149, (1975), pp. 439-442.
Ham et al., "Nutritional Requirements for Clonal Growth of Nontransformed Cells" in, Nutritional Requirements of Cultured Cells, (1978), p. 100.
Spector, "Fatty Acid Glyceride and Phospholipid Metabolism" in, Growth, Nutrition and Metabolism of Cells in Culture, (1972), pp. 257-275.
Toyama Chemical Company, "Vitamin E. and Cyclodextrin Inclusion Compounds" Jpn. Kokai Tokkyo Koho, 81/154,479, (4-1981), pp. 1-3, Chem. Abst., 96:103983.
Szejtli et al., "Enrichment of the Unsaturated Fatty Acid Content in Fatty Acid Mixtures by Formation of Inclusion Complexes" Hung. Teljes., 16,602, (1979), pp. 1-18, Chem. Abst., 92: 22061x.
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