Culture media package

Details Culture media package Culture media package

1989-06-08

1991-01-29

Jones, Larry

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

435299, 435296, C12M 122

Patent

active

049883020

ABSTRACT:
A culture media package comprising a container having a bottom wall and a peripheral side wall which is substantially filled with a culture medium, a removable insert over the top wall and having a portion defining a planar undersurface and a removable protective cover over the container and insert. The container and insert have radially extending flanges which are bonded to one another. The package is made by filling the container with a molten culture medium in a sterile environment, bonding the insert to the container in a sterile environment, inverting the sealed container such that the molten medium contacts the planar surface of the insert permitting any gases and bubbles to rise toward a space adjacent the bottom of the container and maintaining the container with the insert thereon in an inverted position until the medium has solidified. The removable cover may be applied to the sealed package of the container and insert either before the sealed package is inverted or after the medium has solidified. The package is preferably made from continuous molded plastic strips of connected containers and connected inserts which are filled with medium and sealed, severed from one another, thereafter inverted while the culture medium is still molten and permitted to remain in the inverted position until the culture medium has solidified.

REFERENCES:
patent: 4296205 (1981-10-01), Verma
patent: 4534154 (1985-08-01), Gaubert
patent: 4603105 (1986-07-01), Kaplan
patent: 4634676 (1987-01-01), Sapatino
patent: 4797309 (1989-01-01), Krammerer et al.

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