Culture double container and a method of culture

Chemistry: molecular biology and microbiology – Apparatus – Bioreactor

Reexamination Certificate

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Reexamination Certificate

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07964392

ABSTRACT:
A culture container and a method of culture which do not require a plurality of incubators in a case where a plurality of cells of different kinds which like different gas concentrations should be cultured simultaneously.A culture double container1comprises a gas-permeable culture container2having an inlet and an outlet6, a gas-impermeable container3covering the culture container2, and joint portions12and13for holding the culture container2in the gas-impermeable container3in such a manner that space is defined between the culture container2and the gas-impermeable container3. The culture double container1receives gas controlled in its composition in the space defined houses the culture container2and the gas-impermeable container3during culture of cell or micro-organism.

REFERENCES:
patent: 4734372 (1988-03-01), Rotman
patent: 5071760 (1991-12-01), Watanabe et al.
patent: 5362642 (1994-11-01), Kern
patent: 5458593 (1995-10-01), Macabasco et al.
patent: 6670174 (2003-12-01), Smith et al.
patent: 60-27379 (1985-02-01), None
patent: 03-010676 (1991-01-01), None
patent: 03-172169 (1991-07-01), None
patent: 4-71482 (1992-03-01), None
PCT Search Report dated Jun. 14, 2005.
PCT Written Opinion dated Jun. 14, 2005.
Japan Patent Office Action Dated Oct. 12, 2010.

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