Culture dish

Details Culture dish Culture dish

2000-10-18

2001-10-23

Redding, David A. (Department: 1744)

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

C435S305200, C422S105000

Reexamination Certificate

active

06306646

ABSTRACT:

The invention relates to a culture vessel made of plastic for growing cell and tissue cultures according to the preamble of claim
1
. Such vessels have been known for a long time for plant and animal cell cultures. In the past, glass bottles and dishes were primarily used. For one-time use, culture vessels made particularly of thermoplastic materials, e.g., polystyrene, have proven successful. Due to their normally hydrophobic surface, such polystyrene vessels are irradiated, for example, with gamma rays. The culture vessel can be, for example, a Petri dish, a multidish or, for example, also a microtiter plate or a microtest plate. Such culture vessels are described, for instance, in the publication by Lindl and J. Bauer, “Zell- und Gewebekultur” [Cell and Tissue Culture], 3rd Edition, Gustav Fischer Verlag (1994).
Also known are culture vessels whose growing surface is enlarged by elevations. This makes it possible to increase the cell yield 1.7 to 2-fold. Examples of such culture vessels may be found in the publication by Sigma, No. C 5934, of 1998. These vessels, however, present the problem that they substantially complicate microscopic examination. They also make harvesting of the adherent cells more difficult. EPO 552 412 A and EPO 614 967 A show culture vessels that are made as bottles and are respectively provided with a growing surface that has grooves as indentations and ribs as elevations. The distribution of cell and tissue cultures on the ribs is such that microscopic examination is not optimal. Furthermore, the grooves isolate cultures from one another, which adversely affects growth.
The object of the invention is to create a culture vessel of the cited type in which the cells are optically better accessible, are easier to harvest, and which at the same time provides a better cell yield and is nevertheless cost-effective in production.
This object is attained in a generic culture vessel according to claim
1
. Due to this microstructure of the growing surface, the growing surface, but not the volume of the culture medium, is enlarged. Such a microstructure does not complicate harvesting of the adherent cells, nor is microscopic examination made more difficult, since the surface of the cell vessel is substantially flat. Also essential is that these microstructures provide an “in-vivo like” microenvironment for the growing adherent cells. This should substantially contribute to maintaining the state of differentiation and increasing the cell yield.
According to a further embodiment of the invention, the growing surface is provided with elevations and indentations whose height is less than about 100 micrometers. These elevations and indentations are preferably produced in an injection molding process. This type of process makes it possible to produce such elevations having a height of only about 5 nanometers. Preferably, these elevations are regularly arranged and identically shaped. If these elevations are made in the form of truncated pyramids, according to a further embodiment of the invention, regular, flat upper and lower surfaces as well as inclined surfaces are created on which the cells can grow. Since these elevations are very small, the cells are nevertheless situated on substantially the same optical plane for optical examination. In a culture vessel according to the invention, the elevations or indentations are therefore not statistically and randomly distributed but regularly arranged. This also makes it possible to create and test different microstructures for different cell cultures.


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