Culture device and method for eukaryotic cell transfection

Chemistry: molecular biology and microbiology – Apparatus – Mutation or genetic engineering apparatus

Reexamination Certificate

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C435S006120, C435S029000, C435S455000, C435S468000, C435S304100, C435S305100

Reexamination Certificate

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07125709

ABSTRACT:
A cell culture device for transfecting a eukaryotic cell is disclosed. The cell culture/transfection device may be a multiwell plate or slide which has been coated with a metal salt such as CaCl2. Methods of using the cell culture device to transfect mammalian cells and/or to monitor cell transfection are described. Kits are also described which include the cell transfection device, eukaryotic cells to be transformed and nucleic acid for transformation.

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Ausubel et al, eds. Current Protocols in Molecular Biology, 1988, John Wiley & Sons, vol. 1, Unit 9.1, pp. 9.1.1-9.1.4.
Qiagen Inc., “Effecten Transfection Reagent” Product Information, Cat. No. 301425, http://www1.qiagen.com/Products/Transfection/TransfectionReagents/EffecteneTransfection.aspx?ShowInfo=1 accessed Apr. 20, 2005.
International Search Report mailed Feb. 9, 2005 and issued to a related foreign application.

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