Chemistry: molecular biology and microbiology – Apparatus – Inoculator – streaker – or sampler
Patent
1994-10-19
1997-04-01
Yeung, George
Chemistry: molecular biology and microbiology
Apparatus
Inoculator, streaker, or sampler
4353042, 4353092, C12M 100
Patent
active
056164998
ABSTRACT:
Apparatus for testing samples and specimens for microorganisms is provided. The apparatus comprises inoculating means for transferring an inoculum from one medium to another medium; positioning means for positioning the inoculating means in a predetermined position; container means for containing a fluid, semifluid, or solid medium, a sample, and the inoculating means; first aperture means for introducing a sample and a fluid, semi-fluid or solid medium into the container means; second aperture means for introducing the inoculating means into the container means; cap means for closing the second aperture means and for securing the positioning means so that the inoculating means is disposed in a predetermined position in the container means; and, closure means for closing the container means to separate the sample, the fluid medium, and the inoculating means from the outside environment. In another embodiment of this invention, a method is provided for detecting microorganisms in samples or specimens. The method comprises the following steps. First, a sample and an inoculating device is incubated in one or more fluid, semi-fluid or solid media for a pre-selected time at a pre-selected temperature. Second, the inoculating device is removed from the medium in which it has been incubated, and is then used to inoculate at least one other medium, which may be a fluid, semifluid, or solid medium. Next, the inoculated medium is incubated and then tested in accordance with the tests for the microorganism for which the sample or specimen is being tested.
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Eckner Karl F.
Ryser Elliot T.
Smittle Richard B.
Silliker Laboratories Group, Inc.
Yeung George
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