Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-05-08
1992-08-04
Nucker, Christine
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435822, 435 732, 435975, 435 6, 5303871, 5303913, 5303895, 436518, C12Q 104, C12Q 100, C12Q 168, G01N 3353
Patent
active
051358514
ABSTRACT:
Novel autonomously replicating biological particles resembling bacteria and having most surprising properties were discovered from cell culture sera and other biological samples alleged to be sterile according to the current testing methods. These slowly growing agents named Nanobacteria are smaller than any known cell-walled bacteria. They pass through sterile filters, even with pore sizes smaller than their diameter. They cannot be cultured on any standard microbiological media. With the isolation and detection methods provided here they are commonly detectable in animal or human serum. This patent holds for methods of their culture, detection, purification, and elimination and described the necessary reagents for that.
Autonomously replicating particles can be cultured in RPMI 1640, or in DMEM, or in other cell culture media. Optimal growth can be obtained by supplementing the culture medium with 10-20% sterile fetal bovine serum. Addition of small amounts of D,L or L selenomethionine together with nucleotide precursors may improve growth. Culture is started by addition of the test sample to the medium in a cell culture vial which is thereafter incubated under standard mammalian cell culture conditions for at least 15 days. Biological samples are preferably sterile-filtered before culture through 0.22 micron filters. The growth of Nanobacteria, if present, can be seen using microscopy at high magnification. The organisms can be made more visible by DNA staining and immunostaining done either separately or simultaneously to a fixed preparation. Nanobacteria can be cultured without mammalian cells, but co-culture together with an adherent cell line like 3T6 is useful because 3T6 cells can take Nanobacteria inside the cells. This aids the staining of the preparations. Intracellular agents are not lost during fixation and staining.
Nanobacterial antigens can be prepared by specific culture, harvest, purification and solubilization methods. Immunization of rabbits with the solubilized antigen (treatment with proteinase K and with 1 N HCI) produces highly specific antibodies to Nanobacteria. Gamma-irradiation of culture serum at 2.5-4.0 megarads, preferably in addition with treatment using solid-phase bound antibodies enables preparation of Nanobacteria-free serum. Use of this serum creates sterile culture medium for the culture and detection of Nanobacteria. Double staining combining Hoechst No. 33258 stain and immunofluorescence specifically distinguishes Nanobacteria from other cell culture contaminants.
REFERENCES:
Preston Activation Thermodynamics Enhancement of Adsorption to Filter's Masters Thesis, University of Florida 1985, pp. 69-72.
Jawetz et al. Review of Medical Microbiology, 17th Ed. Appleton & Lang, Los Altos Calif. 1987 pp. 306-309.
Roszak et al. Survival Strategies in the Natural Environment, Microbiol. Rev. 51:365-379 Sep. 1987.
Lee et al. A Rapid Multicolor Western Blot. J. Immuno. Chem. 106:27-30, 1988.
Kajander et al. Cell Wall Detective Bacteria Contaminate Cell Culture, Clin. Res. 37(2) 432A Apr. 28-May 1, 1989.
Nucker Christine
Preston David R.
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