Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;... – Culture – maintenance – or preservation techniques – per se
Patent
1995-08-03
1998-05-12
Lankford, Jr., Leon B.
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
Culture, maintenance, or preservation techniques, per se
435410, 435420, 435431, C12N 500
Patent
active
057504016
ABSTRACT:
The present invention relates to a growth medium for the sprouting, multiplication and rooting of bamboo nodal buds from mature bamboo species, via plant tissue culture method, which method comprises a multistage culturing process by collecting a suitable explant from the best bamboo clumps which is first cultured on a solid medium, that induces sprouting of the dormant buds. The sprouts are then further multiplied in an aqueous medium without any solidification. After a certain period, when shoots have been multiplied sufficiently under a proper photoperiod of light and dark, the shoots are transferred to the medium containing low concentration of salts and hormones. Culturing to this point is carried out under subdued light or darkness. Subsequent shifting to hormone free medium after a particular time, the time ranges between 24 hours to 96 hours, develops root initiation. The plantlets thus formed may then be transferred to soil for further growth.
REFERENCES:
patent: 5334530 (1994-08-01), Woods et al.
A Revised Medium For Rapid Growth And Bio . . . Cultures: Murashige etal: Physiol. Plant., 15, 1962: pp. 473-497.
Mascarenhas Anthony Francis
Nadgauda Rajani Satish
Nagarqala Nazifa Najmuddin
Parasharami Varsha Anil
Phadke Chandrashekhar Hari
C.S.I.R.
Lankford , Jr. Leon B.
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