Cultivation of transformed microorganisms

Details Cultivation of transformed microorganisms Cultivation of transformed microorganisms

1990-05-07

1994-01-18

Wax, Robert A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43525233, 4353201, 435849, 536 232, 536 237, 536 241, 935 29, 935 40, 935 41, 935 43, C12N 121, C12N 1511, C12N 1570, C12N 1571

Patent

active

052799514

ABSTRACT:
A process for cultivating a microorganism transformed with a recombinant plasmid at least containing (a) a DNA fragment containing a promoter and a regulator gene tnaC located downstream of the promoter in a tryptophanase operon (tna), and (b) a DNA fragment containing a desired structural gene which can be expressed by the promoter, which comprises cultivating the transformed microorganism in a culture medium while adding glucose as a carbon source continuously or intermittently so that the concentration of glucose is maintained within the range of 0.01 to 0.3%, and thereby allowing the desired structural gene to be expressed in the microorganism.

REFERENCES:
Botsford, J. L. et al. J. Bacteriol. 105:303-312 (1971).
Zaffaroni et al., Agr. Biol. Chem., 38(7) pp. 1335-1342 (1974).
Nichols, B. P. et al. Methods in Enzymology 101:155-164 (1983).

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