Cultivation of Paecilomyces sp. using silkworms

Details Cultivation of Paecilomyces sp. using silkworms Cultivation of Paecilomyces sp. using silkworms

1997-12-05

1999-08-17

Marx, Irene

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

435932, C12M 114

Patent

active

059393106

ABSTRACT:
Disclosed herein is a cultivation of Paecilomyces sp. which has various efficacies such as anti-tumor and reinforcement of immunity, but could not be used wide in human because of relatively limited supply. The artificial cultivation of Paecilomyces sp. comprises steps of (a) inoculating a dilution of spores of Paecilomyces sp. diluted with water to a concentration of 10.sup.7 .about.10.sup.8 spores/ml into a larva of 4th.about.5th instar of silkworm at a temperature of 26.about.30.degree. C. and a humidity of about 90% or more; and (b) inducing fruitbodies of Paecilomyces sp. by maintaining of the larvae at a temperature of 15.about.30.degree. C. and a humidity of about 90% or more. In particular, Paecilomyces japonica (KCCM-10117), Paecilomyces sp. J300 (KCCM-10116) and Paecilomyces farinosa (KCCM-10115).

REFERENCES:
Aoki et al., "The pathogenicity to the silkworm and taxonomic consideration on some muscardine fungi", J. Sericult. Sci. Japan, 1975, 44(5), 365-370.
James et al., "Susceptibility of the convergent lady beetle Coleoptera coccinellidae to four entomogenous fungi", Environmental Entomology, 1994,23(1), 190-192.
Agudelo et al., "Mass production, infectivity and field application studies with the entomogenous fungus, Paecilomyces farinosus", Journal of Invertebrate Pathology, 1983, 42, 124-132.
Ndoye M., "Susceptibility to various entomophagous fungi (Fungi Imperfecti) and development of mycosis by Beauveria bassiana in larvae and pupae of Chilo suppressalis", Bull Inst Foundam Afr Noire Ser A Sci Nat, 1977, 39(2), 303-317.

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