Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology
Reexamination Certificate
2005-03-11
2008-10-21
Crouch, Deborah (Department: 1632)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Method of regulating cell metabolism or physiology
C435S366000, C435S384000, C435S387000, C435S389000
Reexamination Certificate
active
07439064
ABSTRACT:
The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferring, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than just a feeder layer the medium. Also disclosed are compositions capable of supporting the culture and proliferation of human embryonic stem cells without the need for feeder cells or for exposure of the medium to feeder cells.
REFERENCES:
patent: 5405772 (1995-04-01), Ponting
patent: 5453357 (1995-09-01), Hogan
patent: 5670372 (1997-09-01), Hogan
patent: 5690926 (1997-11-01), Hogan
patent: 5750376 (1998-05-01), Weiss et al.
patent: 5817773 (1998-10-01), Wilson et al.
patent: 5843780 (1998-12-01), Thomson
patent: 6200806 (2001-03-01), Thomson
patent: 6245566 (2001-06-01), Gearhart et al.
patent: 7005252 (2006-02-01), Thomson
patent: 7297539 (2007-11-01), Mandalam et al.
patent: 2002/0081724 (2002-06-01), Carpenter et al.
patent: 2002/0137204 (2002-09-01), Carpenter et al.
patent: 2003/0017589 (2003-01-01), Mandalam et al.
patent: 2003/0073234 (2003-04-01), Amit et al.
patent: 2003/0190748 (2003-10-01), Thomson
patent: 2004/0235159 (2004-11-01), Mandalam et al.
patent: 2008/0020458 (2008-01-01), Mandalam et al.
patent: 197 56 864 (1997-12-01), None
patent: WO 97/47734 (1997-12-01), None
patent: WO 98/30679 (1998-07-01), None
patent: WO 99/20740 (1999-04-01), None
patent: WO 99/20741 (1999-04-01), None
patent: WO 00/68359 (2000-11-01), None
patent: WO 01/66697 (2001-09-01), None
patent: WO 03/020920 (2003-03-01), None
patent: WO 2004/099394 (2004-11-01), None
Amit, M., et al., “Feeder Layer- and Serum-Free Culture of Human Embryonic Stem Cells,” Biology of Reproduction 70:837-845 (2004).
Li, Y., et al., “Expansion of Human Embryonic Stem Cells in Defined Serum-Free Medium . . . ,” Biotechnology and Bioengineering, Wiley & Sons 91:688-698 (2005).
Beattie, G.M., et al., “Activin A maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers,” Stem Cells 23:489-495 (2005).
Boilly, B., et al., “FGF signals for cell proliferation and migration through different pathways,” Cytokine & Growth Factor Reviews 11:295-302 (2000).
Clark, A.J., et al., “Germ line manipulation: applications in agriculture and biotechnology,” Transgenic Animals (1992).
Gleveland, W.L., et al., “Routine large-Scale production of Monoclonal Antibodies in a Protein-Free Culture Medium,” J. of Immunological Methods 56:221-234 (1983).
Cruz, Y.P., et al., “Origin of Embryonic and Extraembryonic Cell Lineages in Mammalian Embryos,” Embryonic and Extraembryonic Cell Lineages 147-204 (1991).
Froud, S.J., “The Development, Benefits and Disadvantages of Serum-Free Media,” Dev. Biol. Stand. Basel, Karger 99:157-166 (1999).
Gearhart, J., “Cell Biology: New Potential for Human Embryonic Stem Cells,” Science 282:1061-1052 (1998).
Goldsborough, M.D., et al., “Serum-Free Culture of Murine Embryonic Stem (ES) Cells,” Focus 20:8-121998).
Klimanskaya, I., et al., “Human embryonic stem cells derived without feeder cells,” The lancet 365:1636-1641 (2005).
Mummery, C.L., et al., “Fibroblast growth factor-mediated growth regulation and receptor expression in . . . ,” Biochem-Biophys-Res-Commun. 191-188-95 (1993).
Nichols, J., et al., “Establishment of germ-line-competent embryonic stem (ES) cells using differentiation inhibiting activity,” Development 110:1341-1348 (1990).
Ornitz, D.M., et al., “Receptor Specificity of the Fibroblast Growth Factor Family,” J. of Biological Chemistry 271:15292-15297 (1996).
Pease, S., et al., “Isolation of Embryonic Stem (ES) Cells in media Supplemented with Recombinant Leukemia Inhibitory Factor (LIF),” Developmental Biology 141:344-32 (1990).
Piedrahita, J.A., et al., “On the Isolation of Embryonic Stem Cells: Comparative Behavior of Murine, Porcine and Ovine Embryos,” Theriogenology 34:879-901 (1990).
Rossant, J., et al., “In search of the tabula rasa of human cells,” Nature Biotechnology 17:23-24 (1999).
Thomson, J.A., et al., “Isolation of a primate embryonic stem cell line,” Proc. Natl. Acad. Sci. USA 92:7844-7848 (1995).
Thomson, J.A., et al., “Embryonic Stem Cell Lines Derived from Human Blastocysts,” Science Magazine 282:1145 (1997).
Xie, M-H., et al., “FGF-19, A Novel Fibroblast Growth Factor with Unique Specificity for FGFR4,” Cytokine 11:729-735 (1999).
Xu, C., et al., “Feeder-free growth of undifferentiated human embryonic stem cells,” Technical Report 971-974 (2001).
Yamaguchi, T.P., et al., “Expression of the Fibroblast Growth Factor Receptor FGFR-1/flg during Gastrulation and Segmentation in the Mouse Embryo,” Developmental biology 152:75-88 (1992).
Levenstein Mark
Thomson James A.
Crouch Deborah
Quarles & Brady LLP
Wicell Research Institute, Inc.
LandOfFree
Cultivation of human embryonic stem cells in the absence of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cultivation of human embryonic stem cells in the absence of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cultivation of human embryonic stem cells in the absence of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3988584