Crystals of hepatitis C virus helicase or fragments thereof...

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof

Reexamination Certificate

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C435S007100, C435S183000, C514S002600, C514S012200

Reexamination Certificate

active

07438920

ABSTRACT:
The invention relates to the X-ray crystal structure of the hepatitis C virus helicase domain. More specifically, the invention relates to crystallized complexes of HCV helicase and an oligonucleotide, to crystallizable compositions of HCV helicase and an oligonucleotide and to methods of crystallizing an HCV helicase-oligonucleotide complex. The invention further relates to a computer programmed with the structure coordinates of the HCV helicase oligonucleotide binding pocket or the HCV helicase nucleotide triphosphate pocket wherein said computer is capable of displaying a three-dimensional representation of that binding pocket.

REFERENCES:
patent: WO 97/12043 (1997-04-01), None
D.G. Brown et al., “Crystallography in the Study of Protein-DNA Interactions”,Methods in Molecular Biology, 56 pp. 293-318 (1996).
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J. Jancarik et al., “Sparse Matrix Sampling: A Screening Method for Crystallization of Proteins”,J. Appl. Cryst., 24, pp. 409-411, (1991).
A. Kajihara et al., “Protein Modelling Using a Chimera Reference Protein Derived From Exons”,Protein Eng'g, 6, pp. 615-620 (1993).
K.A. Morgenstern et al., “Polynucleotide Modulation of the Protease, Nucleoside Triphosphatase, and Helicase Activities of a Hepatitis C Virus NS3-NS4A Complex Isolated from Transfected COS Cells”,J. of Virology, 71, pp. 3767-3775 (1997).
A.J. Russell et al., “Rational Modification of Enzyme Catalysis by Engineering Surface Charge”,Nature, 328, pp. 496-500 (1987).
U. Uhlin et al., “Crystallization and Crystallographic Investigations of Ribonucleotide Reductase Protein R1 FromEscherichia coli”, FEBS, 336(1), pp. 148-152 (1993).
N. Yao et al., “Structure of the Hepatitis C Virus RNA Helicase Domain”,Nature Structural Biology, 4, pp. 463-467 (1997).

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