Crystallized N-terminal domain of influenza virus matrix protein

Details Crystallized N-terminal domain of influenza virus matrix protein Crystallized N-terminal domain of influenza virus matrix protein

1997-08-01

2000-07-18

Mosher, Mary E.

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435236, 435239, 435 5, 530350, 378 74, 378 73, 4242041, 4242061, C12N 700, C12N 704, C07K 1411, C12Q 170

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06090609&

ABSTRACT:
The matrix protein, M1, of influenza virus strain A/PR/8/34 has been purified from virions and crystallized. The crystals consist of a stable fragment (18 Kd) of the M1 protein. X-ray diffraction studies indicated that the crystals have a space group of P3.sub.t 21 or P3.sub.2 21. Vm calculations showed that there are two monomers in an asymmetric unit. A crystallized N-terminal domain of M1, wherein the N-terminal domain of M1 is crystallized such that the three dimensional structure of the crystallized N-terminal domain of M1 can be determined to a resolution of about 2.1 .ANG. or better, and wherein the three dimensional structure of the uncrystallized N-terminal domain of M1 cannot be determined to a resolution of about 2.1 .ANG. or better. A method of purifying M1 and a method of crystallizing M1. A method of using the three-dimensional crystal structure of M1 to screen for antiviral, influenza virus treating or preventing compounds. A method of using the three-dimensional crystal structure of M1 to screen for improved binding to or inhibition of influenza virus M1. The use of the three-dimensional crystal structure of the M1 protein of influenza virus in the manufacture of an inhibitor of influenza virus M1. The use of the three-dimensional crystal structure of the M1 protein of influenza virus in the screening of candidates for inhibition of influenza virus M1.

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