Crystal structures of T. foetus inosine monophosphate...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

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C435S183000, C536S028700

Reexamination Certificate

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07083961

ABSTRACT:
The invention provides a crystalline complex containingT. foetusIMPDH in complex with inosine monophosphate (IMP), the complex specified by disclosed atomic coordinates. Also provided are crystalline complexes of containingT. foetusIMPDH with both inosine monophosphate (IMP) and mycophenolic acid, with both xanthosine monophosphate (XMP) and mycophenolic acid, with both xanthosine monophosphate (XMP) and nicotinic adenine dinucleotide (NAD), with ribovirin (1-β-D-ribofuranosyl-1,2,4-triazole-3-carboxamide, and with both ribovirin and mycophenolic acid, each complex specified by disclosed atomic coordinates. Also provided by the invention are the atomic coordinates for these complexes. Further provided by the invention are methods for identifying modulations of IMPDH that employ the atomic coordinates of the invention.

REFERENCES:
Prosise, G.L., Crystal Structure ofTritrichomonas foetusinosine monophosphate dehydrogenase in complex with the inhibitor ribavirin monophosphate reveals a catalysis-dependent ion-binding site. 2002. Jrnl of Biol Chem. 277(52):50654-59.
Beck et al., “Cloning, sequencing, and structural analysis of the DNA encoding inosine monophosphate dehydrogenase (EC 1.1.1.205) fromTritrichomonas foetus,” Exp. Parasitol78(1):101-112 (1994).
Brunger et al., “Crystallography & NMR system: A new software suite for macromolecular structure determination,”Acta. Crystallogr D. Biol. Crystallogr. 54 (Pt 5):905-21 (1998).
Chin et al., “Isolation, sequencing and expression of the gene encoding hypoxanthine-guanine-xanthine phosphoribosyltransferase ofTritrichomonas foetus,” Mol. Biochem. Parasitol. 63(2):221-9 (1994).
Colby et al., “Crystal structure of human type II inosine monophosphate dehydrogenase: implications for ligand binding and drug design,”Proc. Natl. Acad. Sci. U S A96(7):3531-6 (1999).
Digits et al., “Kinetic mechanism ofTritrichomonas foetusinosine 5′-monophosphate dehydrogenase,”Biochemistry38(8):2295-306 (1999).
Digits et al., “Drug selectivity is determined by coupling across the NAD+ site of IMP dehydrogenase,”Biochemistry39(7):1771-7 (2000).
Hager et al., “Recombinant Human inosine monophosphate dehydrogenase type I and type II proteins. Purification and characterization of inhibitor binding,”Biochem Pharmacol. 49(9):1323-9 1995.
Jones et al., “Improved methods for building protein models in electron density maps and the location of errors in these models,”Acta, Crytallogr A.47 (Pt 2):110-9 (1991).
Kerr et al., “Monovalent cation activation inEscherichia coliinosine 5′-monophosphate dehydrogenase,”Arch. Biochem. Biophys. 375(1):131-7 (2000).
Kuntz et al., “A geometric approach to macromolecule-ligand interactions,”J. Mol. Biol.161(2):269-88 (1982).
Laskowski et al., “Computer Programs,”J. App. Cryst.26:283-291 (1993).
Luecke et al., “Tritrichomonas foetus: a strategy for structure-based inhibitor design of a protozoan inosine-5′-monophosphate dehydrogenase,”Exp Parasitol. 87(3):203-11 (1997).
Metz et al., “Inosine-5′-monophosphate dehydrogenase is required for mitogenic competence of transformed pancreatic beta cells”,Endocrinology142(1):193-204 (2001).
Schuller et al., “The allosteric ligand site in the Vmax-type cooperative enzyme phosphoglycerate dehydrogenase,”Nat. Struct. Biol.2(1):69-76(1995).
Sintchak et al., “Structure and mechanism of inosine monophospate dehydrogenase in complex with the immunosuppressant mycophenolic acid”,Cell. 85(6):921-30 (1996).
Stehle et al., “Structure of NADH peroxidase fromStreptococcus faecalis10C1 refined at 2.16 A resolution”,J. Mol. Biol.221(4):1325-44 (1991).
van Aalten et al, “PRODRG, a program for generating molecular topologies and unique molecular descriptors from coordinates of small molecules”,J. Comput. Aided Mol. Des.10(3):255-62 (1996).
Verham et al., “Purification, characterization, and kinetic analysis of inosine 5′-monophosphate dehydrogenase ofTritrichomonas foetus”, Mol Biochem Parasitol.24(1):1-12 (1987).
Whitby et al., “Crystal structure ofTritrichomonas foetusinosine-5′-monophosphate dehydrogenase and the enzyme-product complex”,Biochemistry. 36(35):10666-74 (1997).
Wilson et al., “Amplification and molecular cloning of the IMP dehydrogenase gene ofLeishmania donovani”, J. Biol. Chem.266(3):1665-71 (1991).

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