Crystal of a protein-ligand complex containing an N-terminal tru

Details Crystal of a protein-ligand complex containing an N-terminal tru Crystal of a protein-ligand complex containing an N-terminal tru

1997-06-13

1999-02-16

Patterson, Jr., Charles L.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

530350, G01N 3050, C07K 1400

Patent

active

058720111

ABSTRACT:
A detailed three-dimensional structure for the least abundant of the general translation initiation factors in eukaryotes, eIF4E, complexed with a ligand is disclosed. The novel N-terminal truncated eIF4Es which were constructed so as to omit a significant portion of the flexible N-terminal tail of the eIF4E are also part of the present invention. In addition, the crystals of the protein-ligand complexes containing the N-terminal truncated eIF4Es are also included. Furthermore, methods of identifying antagonists of the eIF4E protein which can be used to regulate protein synthesis in cells are also disclosed.

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