Cryptic regulatory elements obtained from plants

Details Cryptic regulatory elements obtained from plants Cryptic regulatory elements obtained from plants

2007-12-04

2007-12-04

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S320100, C435S468000, C536S023100

Reexamination Certificate

active

10437261

ABSTRACT:
T-DNA tagging with a promoterless β-glucuronidase (GUS) gene generated transgenicNicotiana tabacumplants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.

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